天台乌药的组织培养与快速繁殖  

Tissue culture and rapid propagation of Lindera aggregate (Sims) Kosterm

作　　者：陈珍[1] 孙骏威[2] 许海丹[3] 钱宝英[1] 鲍思伟[1] 

机构地区：[1]台州学院生命科学学院,浙江临海317000 [2]中国计量学院生命科学学院,浙江杭州310018 [3]台州学院医药化工学院,浙江临海317000

出　　处：《浙江农业学报》2012年第2期243-246,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省台州市科技计划项目(071KY07)

摘　　要：以药用植物天台乌药当年生嫩茎为外植体,新芽萌动后取材,研究不同培养基对其组织培养和植株再生的影响。结果表明,最适的不定芽增殖培养基为MS+6-BA 2.0～3.5 mg.L-1+NAA 0.2 mg.L-1+Na2S2O31.25%(蔗糖3%),最佳的生根培养基为1/4 MS+IBA 1.0 mg.L-1(不加肌醇,蔗糖2%)。The effects of different culture media on tissue culture and plant regeneration of medical plant Lindera aggregate（Sims） Kosterm were investigated with the explants of young stems with buds sprouting.The results showed that the appropriate culture medium for adventitious buds multiplication was MS basal culture medium supplemented with 6-BA 2.0～3.5 mg·L-1,NAA 0.2 mg·L-1 and Na2S2O31.25%（3% sucrose）.The most suitable medium for root regeneration was 1/4 MS culture medium added with IBA 1.0 mg·L-1（2% sucrose,without inositol）.

关 键 词：乌药(Lindera AGGREGATE (Sims) Kosterm) 组织培养 快速繁殖 

分 类 号：S567.19[农业科学—中草药栽培]