Acaricidal activities of whole cell suspension,cell-free supernatant,and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.)  

作　　者：Prapassorn BUSSAMAN Chirayu SA-UTH Paweena RATTANASENA Angsumarn CHANDRAPATYA 

机构地区：[1]Biocontrol Research Unit,Department of Biotechnology,Mahasarakham University,Maha Sarakham 44150,Thailand [2]Department of Entomology,Kasetsart University,Chatuchak,Bangkok 10900,Thailand

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2012年第4期261-266,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. RTA 4880006)supported by the Thailand Research Fund,Kasetsart University and Mahasarakham University

摘　　要：Xenorhabdus bacterium has been used as a biological control agent against Luciaphorus sp., a mushroom mite endemic in Thailand. To develop an effective formulation ofXenorhabdus stokiae, treatments using different parts of X. stokiae isolate PB09 culture, including whole cell suspension, cell-free supernatant, and crude cell extract, were performed. The results show that different parts ofX. stokiae isolate PB09 culture could induce variable effects on mite mortality and fecundity. Application with cell-free supernatant of X. stokiae culture resulted in both the highest mite mortality rate [（89.00＋3.60）%] and the lowest mite fecundity [（41.33_＋23.69） eggs/gravid female]. Whole cell suspen- sion of X. stokiae isolate PB09 culture was found to be slightly less effective than its cell-free supernatant, suggesting that X. stokiae was more likely to release its metabolites with acaricidal activities to the surrounding culture media. Crude cell extract of X. stokiae was not effective against mites. Cell-free supematant of X. stokiae isolate PB09 was the most effective biological control agent and it could be conveniently used in future formulations instead of live bacteria.Xenorhabdus bacterium has been used as a biological control agent against Luciaphorus sp.,a mushroom mite endemic in Thailand.To develop an effective formulation of Xenorhabdus stokiae,treatments using different parts of X.stokiae isolate PB09 culture,including whole cell suspension,cell-free supernatant,and crude cell extract,were performed.The results show that different parts of X.stokiae isolate PB09 culture could induce variable effects on mite mortality and fecundity.Application with cell-free supernatant of X.stokiae culture resulted in both the highest mite mortality rate [(89.00±3.60)%] and the lowest mite fecundity [(41.33±23.69) eggs/gravid female].Whole cell suspen-sion of X.stokiae isolate PB09 culture was found to be slightly less effective than its cell-free supernatant,suggesting that X.stokiae was more likely to release its metabolites with acaricidal activities to the surrounding culture media.Crude cell extract of X.stokiae was not effective against mites.Cell-free supernatant of X.stokiae isolate PB09 was the most effective biological control agent and it could be conveniently used in future formulations instead of live bacteria.

关 键 词：XENORHABDUS Luciaphorus Whole cell suspension Cell-free supematant Crude cell extract Mushroom mite 

分 类 号：S436.462[农业科学—农业昆虫与害虫防治]