Spermidine affects the transcriptome responses to high temperature stress in ripening tomato fruit  

作　　者：Lin CHENG Rong-rong SUN Fei-yan WANG Zhen PENG Fu-ling KONG Jian WU Jia-shu CAO Gang LU 

机构地区：[1]Key Laboratory of Horticultural Plant Growth,Development and Biotechnology,Ministry of Agriculture,Department of Horticulture,Zhejiang University,Hangzhou 310029,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2012年第4期283-297,共15页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Basic Research Program (973) of China (No.2009CB119000);the National Natural Science Foundation of China (Nos.31071804 and 30771470);the Zhejiang Provincial Natural Science Foundation (Nos.R3110209 and 2009C32025)

摘　　要：Objective:High temperature adversely affects quality and yield of tomato fruit.Polyamine can alleviate heat injury in plants.This study is aimed to investigate the effects of polyamine and high temperature on transcriptional profiles in ripening tomato fruit.Methods:An Affymetrix tomato microarray was used to evaluate changes in gene expression in response to exogenous spermidine(Spd,1 mmol/L) and high temperature(33/27 °C) treatments in tomato fruits at mature green stage.Results:Of the 10 101 tomato probe sets represented on the array,127 loci were differentially expressed in high temperature-treated fruits,compared with those under normal conditions,functionally characterized by their involvement in signal transduction,defense responses,oxidation reduction,and hormone re-sponses.However,only 34 genes were up-regulated in Spd-treated fruits as compared with non-treated fruits,which were involved in primary metabolism,signal transduction,hormone responses,transcription factors,and stress re-sponses.Meanwhile,55 genes involved in energy metabolism,cell wall metabolism,and photosynthesis were down-regulated in Spd-treated fruits.Conclusions:Our results demonstrated that Spd might play an important role in regulation of tomato fruit response to high temperature during ripening stage.Objective： High temperature adversely affects quality and yield of tomato fruit. Polyamine can alleviate heat injury in plants. This study is aimed to investigate the effects of polyamine and high temperature on transcriptional profiles in ripening tomato fruit. Methods： An Affymetrix tomato microarray was used to evaluate changes in gene expression in response to exogenous spermidine （Spd, 1 mmol/L） and high temperature （33/27 ~C） treatments in tomato fruits at mature green stage. Results： Of the 10101 tomato probe sets represented on the array, 127 loci were differentially expressed in high temperature-treated fruits, compared with those under normal conditions, functionally characterized by their involvement in signal transduction, defense responses, oxidation reduction, and hormone re- sponses. However, only 34 genes were up-regulated in Spd-treated fruits as compared with non-treated fruits, which were involved in primary metabolism, signal transduction, hormone responses, transcription factors, and stress responses. Meanwhile, 55 genes involved in energy metabolism, cell wall metabolism, and photosynthesis were down-regulated in Spd-treated fruits. Conclusions： Our results demonstrated that Spd might play an important role in regulation of tomato fruit response to high temperature during ripening stage.

关 键 词：Solanum Ivcooersicum L.. Soermidine. Hioh temperature. Microarrav. Gene exDression 

分 类 号：S641.2[农业科学—蔬菜学] Q78[农业科学—园艺学]