色素痣脱细胞真皮基质制备方法的实验研究  

Preparation of acellular dermal matrix of pigmented nevus

作　　者：崔鲜月[1] 姜晓丽[1] 张晨[1] 

机构地区：[1]大连大学附属新华医院整形外科,辽宁大连116021

出　　处：《中国美容整形外科杂志》2012年第4期244-247,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 通过对色素痣组织的脱细胞处理,制备一种能够避免异种和同种异体脱细胞真皮基质缺点的自体脱细胞真皮,为自体组织回植修复组织缺损提供实验依据.方法 将手术切除的1例患者的色素痣组织,切取2cm×2cm大小的色素痣25块,置于0.25% DispaseⅡ试剂中,室温下消化24h,去除表皮后,随机分成5个组,分别置于0.25%、0.5%、1.0%、2.5%、5.0%Triton X-100试剂中室温下消化.于不同的作用时间,取标本进行光镜、扫描电镜、透射电镜等检测.结果 色素痣组织在0.25%DispaseⅡ室温下作用24h,去除表皮,再分别用不同浓度的Triton X-100处理48～72h,可有效地去除色素痣组织中的痣细胞及其他所有细胞成分.光镜下胶原纤维粗细均匀,排列规整,无明显变性.扫描电镜观察可见起伏的皮肤纹理及天然毛孔结构,胶原纤维完整而连续,呈规律排列,相互交织成疏松立体网状结构.透射电镜观察胶原纤维粗细均匀,结构清晰,排列规整,可见细胞脱除后留下的腔隙,未见任何细胞碎片的残留.结论 用Triton X-100处理经DispaseⅡ消化去表皮的色素痣组织,当Triton X-100浓度超过0.5%以及Triton X-100作用时间超过48h,可有效地去除色素痣组织中的痣细胞及其他所有细胞成分,进一步增加Triton X-100浓度和脱细胞时间对脱细胞的效果无明显影响.Objective To prepare autogeneic acellular dermal matrix by acellular process of pigmented nevus tissue, providing experimental basis for the tissue defective repair by replantation with autogenic tissue, neth otis Totally 25 pieces （2 cm ×2 cm） of pigmented nevus tissues obtained from the surgical removal were digested in 0.25% Dispose U solution for 24 hours under room temperature to remove epidermis, and then they were divided randomly into 5 groups. These pieces in each group were immerged into 0.25%, 0.5%, 1.0%, 2.5%, 5.0% Triton X-100 solutions for digestion under room temperature. Samples were taken at different time for examinations of light microscopy, scanning electron microscopy, transmission electron microscopy. Results After treated with 0.25% Dispase Ⅱ solution for 24 hours digestion and different concentrations of Triton X-100 for 48 to 72 hours, nevus cells and all other cellular components of pigmented nevus tissues can be effectively removed. The collagen fibers were uniform in thickness, regular in arrangement and no obvious degeneration under light microscopy. The skin texture, natural pore structure, complete and continuous collagen fibers interwovening into a loose three-dimensional network structure can be seen under scanning electron microscopy. Under transmission electron microscopy, the collagen fibers were uniform in thickness, clear in structure, regular in arrangement. There were cavities left by cellular removal, without any residual cell debris. Conclusion In this experiment, when concentration of Triton X-100 was above 0. 5% and processing time was more than 48 hours, nevus cells and all other cellular components of pigmented nevus tissues can be removed effectively. But further increasing the concentration of Triton X-100 and processing time had no significant impact.

关 键 词：色素痣 脱细胞真皮 DispaseⅡ TRITON X-100 

分 类 号：R318.08[医药卫生—生物医学工程]