河北省2009年手足口病病原学研究  

An Etiological study on the hand,foot and mouth disease in 2009 of Hebei Province

作　　者：于秋丽[1] 申悦霞[1] 韩彩芝[1] 谢赟[1] 韩占英[1] 刘立 张艳波[1] 韩旭[1] 李琦[1] 

机构地区：[1]河北省疾病预防控制中心,河北石家庄050021 [2]石家庄市疾病预防控中心

出　　处：《现代预防医学》2012年第8期2026-2028,2031,共4页Modern Preventive Medicine

基　　金：河北省自然基金(CZ010001553)

摘　　要：目的了解2009年引起河北省手足口病的病原谱及分布特征,为制定预防控制措施提供实验依据。方法通过流行病学调查,采集手足口病临床诊断病例的粪便、肛拭子、咽拭子、疱疹液和脑脊液标本,针对肠道病毒(EV)5′端非编码区保守基因序列以及EVVP1至2A区基因序列设计EV通用引物和型特异性引物,通过RT-PCR扩增检测HFMD临床诊断病例的标本,并对病原进行分型。结果采用RT-PCR法共检测HFMD临床诊断病例1990份标本,1296份标本EV阳性,阳性率为65.13%,其中752份标本EV71阳性,452份CA16阳性,1份EV71+CA16阳性,91份其他EV阳性。随机抽取55份其他EV阳性标本经RT-PCR和测序进一步分型,除24份未定型外,共鉴定出31份,其中CA10占18.18%(10/55),CA6占14.55%(8/55),ECO30占9.09%(5/55),ECO6、ECO9各占3.64%(2/55),CA5、CA14、CB5、ECO14各占1.82%(1/55),未定型占43.64%(24/55)。结论 2009年引起河北省手足口病流行的病原体主要为EV71和CA16,同时还有部分CA5、CA6、CA10、CA14、CB5、ECO6、ECO9、ECO14、ECO30等其他型别,引起河北省重症病例的EV主要型别为EV71。OBJECTIVE To investigate the prevalence features of different pathogens of hand,foot and mouth disease（HFMD）,and master and clarify the pathogenic spectrum and the genetic characteristics of HFMD in Hebei Province,so as to provide scientific evidence for the prevention and control of HFMD.METHODS According to epidemiological investigation,stool specimens,anal swab specimens,throat swab specimens,vesicle fluid specimens and cerebrospinal fluid specimens were collected from cases with HFMD.The consensus and type-specific primer pairs were designed respectively according to the conservative gene sequences of 5＇-UTR,the VP1 and 2A gene sequences.Specimens from cases with HFMD were detected by RT-PCR,and the PCR products were identified by agarose gel electrophoresis.RESULTS 1 990 specimens from cases with HFMD were detected by RT-PCR.Among 1 296 specimens with positive EV（65.13%）,752 specimens were EV71（37.79%）,452 were CA16（22.71%）,1 was EV71 ＋ CA16（0.05%）and 91 were the other enterovirus（4.57%）.55 other EV-positive specimens were selected randomly for further analysis by RT-PCR and sequence.31 samples were identified in addition to 24 specimens non typing.From the view of composition,CA10 accounted for 18.18%（10/55）,while CA6 accounted for 14.55%（8/55）,ECO30 accounted for 9.09%（5/55）,and ECO6 and ECO9 accounted for 3.64% respectively,and CA5,CA14,CB5 and ECO14 accounted for 1.82%,respectively,and about 43.64% samples were not typed.CONCLUSION EV71 and CA16 are the primary pathogen of HFMD of Hebei Province in 2009.While there are some CA10,CA5,CA6,CA14,CB5,ECO6,ECO9,ECO14 and ECO30 types.EV71 is the principal pathogen for severe and death cases.

关 键 词：手足口病 肠道病毒 病原谱 RT-PCR 

分 类 号：R372[医药卫生—病原生物学]