高效液相色谱法测定痹痛灵酊中雷公藤内酯醇的含量  

Determination of Triptolide in Bitongling Tincture by HPLC

作　　者：李煌[1] 徐伟[1] 褚克丹[1] 陈立典[2] 张小琴[1] 

机构地区：[1]福建中医药大学药学院,福建福州350108 [2]福建中医药大学康复医学部,福建福州350108

出　　处：《中国中医药信息杂志》2012年第4期51-52,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金："十二五"国家科技支撑计划(2011BAI01B05);福建省科技厅重大专项(2009YZ0001-1-2);福建省科技厅科技创新平台项目(2010Y2004)

摘　　要：目的建立痹痛灵酊中雷公藤内酯醇的含量测定方法。方法采用高效液相色谱法(HPLC),DionexAcclaim 120 C18柱,以甲醇-水(35∶65)为流动相,流速为1.0 mL/min,检测波长为218 nm。结果 HPLC测定雷公藤内酯醇,可达基线分离,雷公藤内酯醇对照品在0.019 2～0.115 2μg内呈线性关系,r=0.999 8,平均回收率为100.76%,RSD=1.85%(n=6)。结论本法简便快速,结果准确,重复性好,可作为该制剂的质量控制方法。Objective To establish the method for the determination of triptolide in Bitongling Tincture.Methods HPLC method was used with Dionex Acclaim 120 C18 column,with methanol-water（35∶65） as the mobile phase,detection wavelength as 218 nm,and flow rate was 1.0 mL/min.Results The concentration of triptolide was linear in the range of 0.019 2-0.115 2 μg.The average recovery was 100.76%（n=6）,and RSD=1.85%.Conclusion This method was sensitive,quick and accurate for the quatity control of the preparation.

关 键 词：痹痛灵酊 高效液相色谱法 雷公藤内酯醇 

分 类 号：R284.1[医药卫生—中药学]