根癌农杆菌介导红颊草莓遗传转化体系的建立  

Establishment of Agrobacterium-mediated Transformation System for 'Benihope' Strawberry

作　　者：古咸彬[1] 王翡[1] 高志红[1] 乔玉山[1] 章镇[1] 林之林[1] 糜林[2] 李金凤[2] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江苏丘陵地区镇江农业科学研究所,江苏句容212400

出　　处：《江西农业学报》2012年第3期11-14,共4页Acta Agriculturae Jiangxi

基　　金：江苏省农业资源开发局项目(2008KJ-046);2011年国家大学生创新训练计划

摘　　要：以草莓品种红颊的叶片为受体,通过对影响遗传转化的一系列因子:潮霉素选择压、抗生素种类和浓度、农杆菌浓度、侵染时间和共培养时间的优化,获得了较高转化效率的体系。结果表明:菌液浓度为OD600=0.4,侵染时间为6 min,选用250 mg/L的Cb作为抑菌抗生素、5 mg/L的潮霉素作为筛选抗生素,共培养时间3 d是一个高效的转化体系,抗性芽诱导率达到25%。Taking the leaves of strawberry cultivar ＇Benihope＇ as experimental acceptor,a higher-efficient genetic transformation system was obtained through the optimization of series of influential factors,such as hygromycin selection stress,type and concentration of antibiotics,agrobacterial concentration,infection time and co-culture time.The results showed that when the agrobacterial concentration was OD600=0.4,the infection time was 6 min,250 mg/L Cb was used as the antibacterial antibiotics,5 mg/L hygromycin was used as the screening antibiotics,and the co-culture time was 3 days,this transformation system was highly efficient,and its inducement rate of resistant shoots reached 25%.

关 键 词：遗传转化 草莓 红颊 

分 类 号：S336[农业科学—作物遗传育种]