亚硒酸钠对小鼠的半数致死量测定  

Determination of median lethal dose of sodium selenite in mice

作　　者：陈爽[1,2] 侯振中[1] 管延杰[1] 李玉侠[1] 韩玉[1] 赵树臣[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]黑龙江农业工程职业学院,哈尔滨150088

出　　处：《东北农业大学学报》2012年第3期91-94,共4页Journal of Northeast Agricultural University

基　　金：黑龙江省教育厅项目(10541023)

摘　　要：研究亚硒酸钠对小鼠的急性毒性作用和半数致死量（LD50）。给70只小鼠分别以2.77、4.3、6.67、10.3、16和24.8 mg.kg-1剂量的亚硒酸钠溶液一次性灌胃,对照组以相同剂量的蒸馏水灌胃,观察7d内小鼠的毒性反应和死亡情况,以改良寇氏法计算LD50,并观察毒性作用。结果表明,亚硒酸钠对小鼠的LD50为10.8 mg.kg-1,其95%可信限为9.0~13.0 mg.kg-1。进而说明,亚硒酸钠有一定的毒性,需要进行毒理学研究,临床应用时应考虑其安全用量。To research the acute toxicity action and the median lethal dose（LD50） of sodium selenite in mice.To observe the toxic reaction and death of mice for seven days,the sodium selenite solution for 70 mice was used as 2.77,4.3,6.67,10.3,16 and 24.8 mg·kg-1 in each group to intragastric administration,distilled water was used in control group,the toxic effect was observed and the LD50 were calculated by Modified Karber＇s method.The dose of LD50 of sodium delenite in mice was 10.8 mg·kg-1,and a 95% confidence range of its LD50 was 9.0-13.0 mg·kg-1.The safety dosage should be considered on clinical application and which should be on toxicology research because the determinate toxicity that sodium selenite had.

关 键 词：亚硒酸钠 小鼠 急性毒性试验 半数致死量 

分 类 号：S854[农业科学—临床兽医学]