川芎嗪注射液对脊柱手术患者自体血回输后IL-2、IL-10及IFN-γ的影响  

Effects of Ligustrazine Injection on IL-2,IL-10,and IFN-γ in Patients Undergoing Spinal Operation after Autologous Blood Transfusion

作　　者：蔡诚毅[1] 马武华[1] 邓恋[2] 张子银[1] 王勇[1] 

机构地区：[1]广州中医药大学第一附属医院麻醉科,广州510405 [2]广东省妇幼保健院麻醉科,广州510165

出　　处：《中国中西医结合杂志》2012年第4期474-477,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省科学技术厅社会发展计划项目(No.2009B030801303);广东省中医药管理局建设中医药强省科研课题(No.2009397)

摘　　要：目的探讨川芎嗪注射液应用于自体血回输对患者细胞免疫功能的调节作用。方法选取60例广州中医药大学第一附属医院脊柱骨科2009年10月-2010年6月择期腰椎后路全椎板切除减压髓核摘除植骨内固定手术患者,随机分为试验组和对照组,每组30例。试验组于收集自体血前30min静脉滴注川芎嗪注射液(2mg/kg),在回收血液的肝素盐水和洗涤盐水内加入川芎嗪注射液(终浓度为0.005%);对照组不予川芎嗪注射液,余处理同试验组。记录两组手术时间、术中出血量及回输血量,采集术前(T1)及回输自体血后1h(T2)、1天(T3)、5天(T4)静脉血,采用ELISA法测定白细胞介素-2(interleukin-2,IL-2)、白细胞介素-10(inter-leukin-10,IL-10)、干扰素-γ(Interferon-γ,IFN-γ)水平,并计算IL-2/IL-10值。结果两组手术时间、术中出血量、回输血量及T1时间点IL-2、IL-10、IFN-γ水平、IL-2/IL-10比较,差异均无统计学意义(P>0.05)。与本组T1时间点比较,两组T2-4IL-2水平降低,T2-3IL-10水平升高、IL-2/IL-10降低,对照组T2-4IFN-γ水平降低,T4IL-2/IL-10降低,T4IL-10水平升高,试验组T4IL-10水平降低,差异均有统计学意义(P<0.05,P<0.01)。试验组T2-4IL-2、IFN-γ水平及IL-2/IL-10高于对照组同期,T2、4IL-10水平低于对照组同期,差异均有统计学意义(P<0.05,P<0.01)。结论川芎嗪注射液应用于自体血回输对细胞因子平衡具有调节作用。Objective To study the regulatory effect of Ligustrazine Injection（LI） on the cellular immune function in patients undergoing autologous blood transfusion（ABT）.Methods Enrolled were 60 patients scheduled for receiving selective lumbar surgery at the Department of Spinal Orthopedics,First Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine during October 2009 to June 2010.They were equally randomized into two groups,the trial group and the control group.LI was given to patients in the trial group by intravenous dripping at the dose of 2 mg/kg 30 min before autologous blood collection.The LI（at the final concentration of 0.005%） was added in the heparin saline solution and the washing saline for recycle blood.No LI was given to patients in the control group.They received the same treatment of the trial group.The operation time,the amount of blood loss and blood transfusion were recorded.Patients＇ venous blood samples were collected for determining cytokines including interleukin-2（IL-2）,interleukin-10（IL-10）,interferon-γ（IFN-γ） by ELISA and calculating IL-2/IL-10 ratio before surgery（T1）,1 h（T2）,1 day（T3）,and 5 days（T4） after ABT.Results There was no statistical difference in the amount of blood loss and blood transfusion,the levels of IL-2,IL-10,IFN-γ,or IL-2/IL-10 at T1 between the two groups（P0.05）.Compared with T1 of the same group,the level of IL-2 decreased at T2-4,IL-10 increased and IL-2/IL-10 decreased at T2-3 in the two groups.The level of IFN-γ decreased at T2-4,IL-2/IL-10 increased at T4,the level of IL-10 decreased at T4 in the control group（P0.05,P0.01）.The level of IL-10 decreased at T4 in the trial group with statistical difference（P0.05,P0.01）.Compared with the control group,the level of IL-2,IFN-γ,and IL-2/IL-10 at T2-4 were obviously higher in the trial group.But the IL-10 level was lower in the trial group than in the control group at T2-4（P0.05,P0.01）.Conclusion The application of LI in ABT had regulatory eff

关 键 词：自体血回输 川芎嗪注射液 细胞因子 免疫功能 

分 类 号：R457.1[医药卫生—治疗学]