柄海鞘共附生细菌的分离培养与系统发育多样性研究  

Isolation and Phylogenetic Analysis of Associated Bacteria From the Styela clava

作　　者：苗婷婷[1] 邢翔[1] 杜宗军[1] 陈冠军[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《海洋科学进展》2012年第1期111-118,共8页Advances in Marine Science

基　　金：国家自然科学基金重点项目--渤海中南部底栖生物生产过程与生物多样性集成研究(40730847);山东大学自主创新基金项目--海洋微生物的资源采集及其次生代谢产物研究(IIFSDU)

摘　　要：通过纯培养和16SrRNA基因序列的相似性比对,并构建系统发育树对威海海域柄海鞘共附生细菌的多样性进行了研究。用2216E培养基从柄海鞘样品中分离到78株细菌,通过形态学特征和TCBS培养基选择性筛选后,选取30株具有代表性的菌株进行了16SrRNA基因测序和基于16SrRNA基因序列的系统发育多样性分析。结果表明,30株菌株分别属于细菌域的4个系统发育类群(Actinobacteria,Bacteroidetes,Firmicutes,Gro-teobacteria)的11个科,11个属。根据16SrRNA基因序列,大多数菌株与其系统发育关系最密切的模式菌株之间存在一定的遗传差异(16SrRNA基因序列相似性为91.82%～98.93%)。其中菌株HQW7,HQYD1,HQWD4,HQE1和HQE2与相关模式菌株的16SrRNA基因最高相似度仅分别为91.82%,92.51%,92.99%,93.52%,93.87%,这5株菌可能代表新的分类单元。威海海域柄海鞘共附生细菌存在着较为丰富的物种多样性和系统发育多样性,并可能存在新的物种。Associated bacteria were isolated from Styela clava collected from the coast of Weihai,China.They were cultured by using conventional culture-dependent method and investigated by using phylogenetic analysis based on 16S rRNA gene sequence comparisons.The 78 isolates were selected from samples on marine 2216 medium（Difco）.Based on morphological characters and weather growing on TCBS selective medium,we selected 30 strains for molecular phylogenetic study using 16S rRNA gene sequences.Our results showed that 30 isolates were members of 11 genera of 11 families in 4 major phylogenetic groups（Actinobacteria,Bacteroidetes,Firmicutes,Proteobacteria）.Most of the strains had certain genetic differences between the closely known type strains（16S rRNA gene sequence similarity were 95.57%~99.88%）.The lower similarity suggested that at least 5 strains represented new species.The results presented above showed that there were abundant species diversity and phylogenetic diversity of bacteria isolated from Styela clava.

关 键 词：柄海鞘 共附生细菌 16S RRNA基因 系统发育分析 细菌多样性 

分 类 号：Q938.1[生物学—微生物学]