白千层枝和叶总黄酮含量的测定  

Determination of Total Flavonoid Content in the Stems and Leaves of Melaleuca leucadendron Linn

作　　者：范超君[1] 鲍长余[1] 陈湛娟[1] 毕和平[1,2] 

机构地区：[1]海南师范大学化学与化工学院,海南海口571158 [2]海南师范大学"省部共建-热带药用植物化学"教育部重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2012年第1期63-65,共3页Journal of Hainan Normal University（Natural Science）

基　　金：海南师范大学国家大学生创新实验项目(101165804)

摘　　要：以芦丁为对照品，NaNO2-Al（NO3）3-NaOH为显色系统，用紫外分光光度法，在498nm波长测定白千层枝和叶中总黄酮的含量。结果显示，其回归方程为A=0.01022C＋0.0165，r=0.9995，吸光度在0-100mg／L范围内呈良好线性关系。测定结果表明，白千层枝和叶中总黄酮的质量分数分别为0.69％和0.63％。A The total flavonoids content in the stems and leaves of Melaleuca leucadendron Linn was determined by spectrophotometry with rutin standard and NaNO2-Al（NO3）3-NaOH system. The regression equation was A=0.01022C＋ 0.0165 （r=0.9995）.The results showed that the mass fractions of total flavonoid in the stems and leaves were 0.69% and 0.63% respectively. The method was stable, accurate, reproducible, highly sensitive and thus was a realistic and practi cal approach for determining flavonoid content of Melaleuca leucadendron linn.

关 键 词：白千层 总黄酮 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]