氦氖激光与紫外线B辐射对小麦幼苗根氮代谢相关酶的影响  

Influence of He-Ne Laser Irradiation and UItraviolet-B Radiation on Nitrgen Metabolism Enzymes in Wheat Seedling Roots

作　　者：李素花[1] 孙永星[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041000

出　　处：《石河子大学学报（自然科学版）》2012年第1期23-27,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30671061);山西省自然科学基金项目(2008011059-1)

摘　　要：为研究He-Ne激光辐照对小麦幼苗根氮代谢相关酶受UV-B辐射损伤后的修复作用,分别采用5mW.mm-2 He-Ne激光辐照、10.08kJ.m2.d1的UV-B辐射及二者组合对临优2018号小麦幼苗进行处理,3d后测定各处理组小麦幼苗根一氧化氮合成酶含量和硝酸还原酶活性的变化。分析结果显示:He-Ne激光辐照可使UV-B辐射后小麦幼苗根一氧化氮合成酶(NOS)含量增加4.7%,硝酸还原酶活力升高1.01倍,显著改善了增强UV-B辐射后硝酸还原酶(NR)活性降低的程度。表明一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗根氮代谢水平的辐射损伤。The wheat seedling roots（Linyou 2018）were exposed to He-Ne laser（5 mW·mm-2）,to the enhanced UV-B radiation（10.08 kJ·m-2·d-1） and to the combined He-Ne laser irradiation and enhanced UV-B radiation.3 d later the changes of NOS（nitric oxide synthases） content and the NRA（nitrate reductase activity） of different treatment groups in different trentment time were measured to test the repair role of laser irradiation.The results show that He-Ne laser irradiation can make the content of NOS increase by 4.7% and nitrate reductase（NR） activity increase by 101%,significantly improving the lower degree of nitrate reductase（NR） activity.It suggests that He-Ne irradiation could partially repair the UV-B radiation damage to the nitrogen meatabolism of wheat seedling roots.

关 键 词：UV-B辐射He-Ne激光小麦 一氧化氮合成酶硝酸还原酶 

分 类 号：S512[农业科学—作物学] Q947[生物学—植物学]