葡萄膜炎患者外周血T细胞TCR BV亚家族克隆性的初步研究  

Study on the T cells of TCR BV CDR3 Lineage Polymorphism in Peripheral Blood of Uveitis Patients

作　　者：魏克娜[1] 黄红艳[2] 张璐[1] 邹红云[3] 余伍忠[3] 焦敏[3] 

机构地区：[1]石河子大学医学院免疫学教研室,石河子832002 [2]兰州军区乌鲁木齐总医院眼科,乌鲁木齐830000 [3]兰州军区乌鲁木齐总医院临床医学研究所,乌鲁木齐830000

出　　处：《石河子大学学报（自然科学版）》2012年第1期51-55,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金(81172840);兰州军区医药卫生科研基金资助项目(CWS10JA21)

摘　　要：为探讨葡萄膜炎患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)TCR BV CDR3谱系特点及多态性,为其免疫发病机制研究提供实验基础。采用RT-PCR扩增TCR BV 26个亚家族CDR3的方法,经免疫扫描谱型技术分析对葡萄膜炎患者PBMC中TCR BV CDR3的谱系漂移情况进行研究。结果显示:(1)5例正常健康人PBMC TCR BV CDR3谱型绝大多数呈正态(或高斯)分布,4例葡萄膜炎患者TCR BV CDR3扫描谱型均出现非正态分布的异常峰型,包括寡峰/寡峰趋势,偏峰和不规则异常峰型;(2)在26个TCR BV亚家族中,不同亚家族异常峰型出现的频率不同,非正态异常峰型出现频率较高的亚家族有BV2和BV17(均为3/4),而BV5.2、BV6、BV15和BV18亚家族均未出现异常峰型;(3)TCR BV2和BV17两个亚家族在HLA-B27阴性的3个患者均出现非正态异常峰型,而在HLA-B27阳性(合并强直性脊柱炎)的患者并未出现异常。葡萄膜炎患者PBMC TCRBV部分亚家族的异常表达可能与该病的免疫发病机理有关,为葡萄膜炎发病机制的进一步研究提供依据。To study T cells lineage polymorphism of TCR BV CDR3 in peripheral blood of uveitis patients so as to provide experimental basis to immune pathogenesis research in uveitis.T cells TCR BV 26 subfamily CDR3 of uveitis patients PBMC were amplified by RT-PCR method,then TCR BV CDR3 lineages polymorphism were analyzed by immunization scanning spectrum.Results：1）Most spectral type of PBMC TCR BV CDR3 in five normal controls showed Gauss distribution,TCR BV CDR3 scanning spectrum of 4 cases uveitis patients all showed abnormal distribution peak,including oligoclonal/oligoclonal trend,skewing peak and irregular abnormal peak;2）Frequencies of abnormal peak type occurrence varied in the 26 TCR BV subfamilies：high frequency abnormal peak type subfamilies were BV2 and BV17（both 3/4）,while BV5.2,BV6,BV15 and BV18 subfamilies had no abnormal peak type;3）TCR BV2 and BV17 two subfamily in three patients with HLA-B27 negative showed abnormal peak type,while HLA-B27 positive（complicating ankylosing spondylitis） patients did not appear abnormal.Uveitis in patients with PBMC TCR BV subfamily expression might be related to immune pathogenesis.This study provides a foundation for further research into the mechanism of uveitis.

关 键 词：葡萄膜炎 T细胞受体 BV亚家族 互补决定区3 免疫扫描谱型分析 克隆性 

分 类 号：R392.9[医药卫生—免疫学] R773.9[医药卫生—基础医学]