16s rDNA文库法分析番石榴果实蝇共生菌组成  

Composition of symbiotic bacteria associated with Bactrocera correcta analyzed by 16s rDNA libraries

作　　者：戴阳[1] 李志红[1] 柳丽君[1] 吴佳教[2] 邓裕亮 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]广东检验检疫技术中心,广州510623 [3]西双版纳出入境检验检疫局,景洪666100

出　　处：《植物保护》2012年第2期60-65,共6页Plant Protection

基　　金：国家"973"计划项目(2009CB119204);国家自然科学基金(30971916)

摘　　要：[目的]探索分析番石榴果实蝇[Bactrocera correcta(Bezzi)]的共生菌组成,为进一步探索共生菌的生理功能以及与宿主的协同进化关系奠定基础。[方法]构建番石榴果实蝇室内种群共生菌的16srDNA文库,BLAST分析其共生菌的组成以及丰度。[结果]室内雄虫共生菌主要为Pseudomonas aeruginosa和Lactococcus lactis,雌虫共生菌主要为Enterobacter属的细菌。[结论]本研究结果证明番石榴果实蝇存在多种共生菌。[Objective] The composition of symbiotic bacteria associated with Guava fruit fly,Bactrocera correcta（Bezzi）,was investigated to provide the basis for further study on physiological function of the symbionts and co-evolution between bacteria and the host.[Method] 16s rDNA libraries of laboratory population for B.correcta were constructed,and BLAST analysis of bacterial composition and abundance was carried out in this study.[Result] The dominant symbionts in B.correcta male adults were Pseudomonas aeruginosa and Lactococcus lactis,while symbiotic bacteria in female adults were Enterobacter bacteria.[Conclusion] Various bacteria are present in the Guava fruit fly,B.correcta.

关 键 词：番石榴果实蝇 16s rDNA文库 共生菌 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]