昆虫生长调节剂和绿僵菌对意大利蝗几丁质酶活力的影响  

The effect of insect growth regulators and Metarhizium anisopliae on the Calliptamus italicus chitinase

作　　者：赵忠伟[1,2] 曹广春[1] 徐光青 吴乐年 张泽华[1] 

机构地区：[1]中国农业科学院植物保护研究所,农业部生物防治重点开放实验室,北京100081 [2]沈阳农业大学植物保护学院,沈阳110161 [3]新疆阿勒泰地区治蝗灭鼠办公室,阿勒泰836500 [4]新疆哈巴河县治蝗办公室,哈巴河836700

出　　处：《植物保护》2012年第2期83-86,共4页Plant Protection

基　　金：公益性行业(农业)科研专项(20090321,201003079);农业部现代农业专项(nycytx-2009-37)

摘　　要：[目的]探明3种昆虫生长调节剂(苯氧威、氟虫脲、灭幼脲)和一种生物农药(绿僵菌189菌株)对意大利蝗3龄蝗蝻几丁质酶活力的影响。[方法]用药剂处理3龄意大利蝗蝗蝻,用分光光度法测定几丁质酶分解几丁质产物N-乙酰氨基葡萄糖的含量。[结果]3种昆虫生长调节剂浓度达到50mg/L时对几丁质酶的抑制作用超过200%;绿僵菌189菌株对意大利蝗几丁质酶活力也有一定程度的抑制,且抑制作用随着孢子浓度的增加而增大,当孢子浓度达到5×107时,意大利蝗几丁质酶的活力仅为3.8U。[结论]昆虫生长调节剂和绿僵菌均抑制意大利蝗几丁质酶活力。[Objective] The effect of three insect growth regulators（fenoxycarb,flufenoxuron,diflubenzuron） and a biological pesticide（189 strains of Metarhizium anisopliae） on the chitinase of the 3rd instars of Calliptamus italicus was studied.[Method] The third instars of the C.italicus were treated by three pesticides and M.anisopliae,respectively.The content of N-acetyl glucosamine produced by chitin decomposition with chitinase was tested by spectrophotometer.[Result] The results showed that all of the four pesticides inhibited chitinase activity.When the concentration of each of the three insect growth regulators reached to 50mg/L,their inhibitory effects on the chitinases were more than 200%.189 strains of M.anisopliae had some inhibitory effects on chitinase of C.italicus.When the spores reached the highest concentration of 5 × 107,the chitinase activity had only 3.8U.[Conclusion] Both insect growth regulators and M.anisopliae can inhibite the chitinase activity of C.italicus.

关 键 词：意大利蝗 昆虫生长调节剂 绿僵菌 几丁质酶 

分 类 号：S482.38[农业科学—农药学]