UPLC法测定酸水解前后化橘红中4种黄酮类成分的含量  

UPLC determination of four flavonoids in exocarpium Citrus grandis before and after acid hydrolysis

作　　者：邓少东[1] 王莲婧[1] 林励[1] 邓韬[1] 林靖然[1] 郑捷[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中华中医药杂志》2012年第4期924-928,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技部"十二五"科技支撑计划项目(No.2011BAI01B02)~~

摘　　要：目的:建立超高效液相色谱法测定酸水解前后化橘红中柚皮苷、野漆树苷、柚皮素、芹菜素的含量。方法:分别以超声法和酸水解法提取样品,超高效液相色谱法检测。采用WATERS ACQUITY UPLC色谱系统,ACQUITY BEH C18色谱柱(2.1mm×50mm,1.7μm),以甲醇-0.1%乙酸水溶液为流动相梯度洗脱,流速0.5mL/min,柱温30℃,检测波长283nm,289nm,337nm。结果:酸水解前可同时测定柚皮苷、野漆树苷、柚皮素、芹菜素的含量,酸水解后柚皮苷、野漆树苷分别转化为柚皮素、芹菜素,4种黄酮与其它成分均达到良好的分离。结论:该方法高效、快速、灵敏,为化橘红的抗氧化活性研究及其新制剂的开发提供参考。Objective： To develop the UPLC method for determination of naringin,rhoifolin,naringenin and apigenin in Exocarpium Citrus grandis before and after acid hydrolysis.Methods： The samples were extracted by ultrasonic and acid hydrolysis.Quantification was performed by UPLC at 30℃ using ACQUITY UPLC BEH C18 column（2.1mm×50mm,1.7μm）,methanol-0.1% acetic acid was applied as mobile phase and DAD detector was set at 283nm,28nm,337nm.The flow rate was 0.5 mL/min.Results： The four flavonoids can be detected before acid hydrolysis.After acid hydrolysis,naringin and rhoifolin translated into naringenin and apigenin,the result showed naringin,rhoifolin,naringenin and apigenin had good separation with other components.Conclusion： This method is efficient,quickly,sensitive and provides a reliable reference to antioxidation research and exploit new preparation for Citrus grandis.

关 键 词：化橘红 超高效液相色谱法 黄酮 盐酸水解 

分 类 号：R284[医药卫生—中药学]