中药复方糖耐康对2型糖尿病KKAy小鼠糖脂代谢及肾脏形态学的影响  

Influence of Tangnaikang Formula on glycolipin metabolism and kidney morphology in type 2 DM KKAy mice

作　　者：吴丽丽[1] 孙文[1] 郭翔宇[2] 刘铜华[1] 杨美娟[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《中华中医药杂志》2012年第4期972-976,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30973909);北京中医药大学创新团队项目~~

摘　　要：目的:探讨中药复方糖耐康对2型糖尿病KKAy小鼠血脂代谢及其早期肾脏病理改变的影响。方法:12周龄雄性KKAy小鼠50只按血糖随机分为模型组、缬沙坦组、糖耐康高、中、低剂量组,另设10只C57BL/6J小鼠为正常组,模型组和正常组给予0.9%氯化钠溶液,其余组给予对应浓度的药液灌胃,每2周检测体质量、空腹血糖,每4周测24h尿蛋白定量,8周后处死,检测血生化指标,观察肾脏病理变化,检测肾组织FN mRNA表达。结果:与同龄正常组小鼠比,模型组小鼠体质量显著增加(P<0.01),各给药组较模型组同期体质量有显著下降(P<0.05,P<0.01):模型组较正常组小鼠的空腹血糖(FBG)、血甘油三脂(TG)、血总胆固醇(TC)、血胰岛素(TNS)显著升高(P<0.01),糖脂代谢紊乱;24h尿蛋白定量、肾重升高(P<0.05),20周龄模型组小鼠肾小球较正常组增大,细胞外膜基质增加,肾小管管腔扩大,蛋白管型明显,半定量分析肾组织病理损伤评分增大,RT-PCR定量分析肾组织FN mRNA表达显著增加(P<0.05);各用药组经缬沙坦、糖耐康治疗后,上述指标均较模型组有明显改善(P<0.05)。结论:糖耐康可调节KKAy小鼠糖脂代谢紊乱,对早期肾脏病理改变有较好的保护作用,抑制FN mRNA沉积改善肾纤维化是其可能的作用机制之一。Objective： To investigate the effects of Tangnaikang on renal pathological changes in the early period of type 2 diabetic KKAy mice model.Methods： 12 weeks＇ male KKAy assigned into five groups randomly： diabetes（DM） group（n=10）,diabetes with high Tangnaikang therapy group（n=10）,diabetes with middle Tangnaikang therapy group（n=10）,diabetes with low Tangnaikang therapy group（n=10）,diabetes with valsartan therapy group（n=10） and C57BL/6J mice.Body weight and FBG was detected once of 2 weeks,24h urine protein quantitative was detected once of 4 weeks.After 8 weeks,All the mouse were sacrificed and the blood serum was obtained,TG,TC,Bun and Scr were detected;Early renal pathological variation was presented by histochemical staining.Kidney FN mRNA expreesion was detected by realtime fluorescence quantitative PCR method.Results： In DM group mice,renal glomerulus area was magnified,GBM was thickening and obvious protein casts were observed compared with those of normal group.Body weight,kidney weight,4h urine protein quantitative,FBG,INS,TG,TC and kidney FN mRNA expreesion was increased.After therapy for 8 weeks,Tangnaikang treatment can alleviate these pathological changes.Conclusion： The results indicate that Tangnaikang can ameliorate the renal pathological variation in the early period,by its effects of decreasing blood glucose and serum lipids.

关 键 词：糖耐康 糖尿病 KKAY小鼠 肾脏病理改变 

分 类 号：R285.5[医药卫生—中药学]