同源四倍体刺槐花序变异表型观察与分析  

Phenotypic Observation and Analysis of Inflorescence Variation of Autotetraploid Robinia pseudoacacia

作　　者：路超[1] 崔彬彬[1,2] 黄禄君[1] 孙鹏[1] 张国君[1,3] 李云[1] 

机构地区：[1]林木育种国家工程实验室北京林业大学生物科学与技术学院,北京100083 [2]保定学院生化系,保定071000 [3]河北科技师范学院园艺科技学院,秦皇岛066004

出　　处：《林业科学》2012年第2期63-68,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31170629;30640036);林业公益行业专项(201104013);北京市农委项目(20080104);北京林业大学研究生基金项目(05jj038)

摘　　要：为揭示同源四倍体花序的遗传变异机制,对同源四倍体刺槐和二倍体刺槐的花序生长进行对比研究。结果显示:与二倍体刺槐相比,同源四倍体刺槐的花序长度没有明显变化,但着生的小花数量明显提高;同源四倍体刺槐花序不同位置小花的发育呈现不同步性;分别从同源四倍体刺槐与二倍体刺槐中克隆出花分生组织决定基因LFY和TFL1的同源基因的部分片段,其同源性分别达到98.44%和98.00%,而去甲基化剂5-acazC能够明显抑制变异花序的形成和促进花序提前开花。A comparative study between autotetraploid and diploid Robinia pseudoacacia was carried out to investigate the mechanism of genetic variations of inflorescence in autotetraploid.The result showed there were no significantly differences in the length of inflorescence between autotetraploid and diploid R.pseudoacacia,but the number of florets was significantly more in autotetraploid than the diploid.The florets of the autotetraploid R.pseudoacacia in different positions showed the remarkable asynchrony.DNA sequences of homologous genes of LFY and TFL1 were cloned from autotetraploid and diploid,and their homology reached to 98.44% and 98.00%,respectively.The treatment with the acazC could observably reduce the inflorescence variation and promote inflorescence bloom prematurely.

关 键 词：刺槐 同源四倍体 花序变异 甲基化 花分生组织决定基因 

分 类 号：S718.42[农业科学—林学] S718.46