基于mtDNA-COI基因序列的针叶小爪螨种群遗传结构影响因素分析  

An Analysis of Factors Affecting Population Genetic Structure of Oligonychus ununguis Based on the Mitochondrial COI Gene Sequences

作　　者：尹淑艳[1] 李波[2] 郭慧玲[3] 李会[1] 李杨[1] 孙绪艮[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]泰安市林业局,泰安271000 [3]泰山风景名胜区管理委员会,泰安271000

出　　处：《林业科学》2012年第2期162-168,共7页Scientia Silvae Sinicae

基　　金：教育部博士点科研基金(20060434)

摘　　要：寄主植物、地理距离、农药胁迫、生境片段化等是影响种群遗传结构和进化的重要因素（Harrison etal.,1996;Hutchinson et al.,1999;Knutsen et al.,2000;罗育发等,2006;褚栋等,2008）。 许多有关昆虫与植物间关系的研究发现植食性昆虫具有通过缩小或扩大其寄主范围或转移到新寄主上的进化潜力（Via,1990）,这种现象可能使种群间产生完全的生殖隔离进而导致与寄主有关的物种形成。In order to understand effects of host plant,geographical distance and pesticide stress on the genetic structure of the spruce spider mite（Oligonychus ununguis）,different populations of the mite were used for analyzing the sequence variation of the mitochondrial cytochrome oxidase I gene（COI） segment.Genetic differentiation was very small in the mites collected from different species of Castanea mollissima,Quercus acutissima,Q.variabilis,Q.dentate,which distributed in a narrow range（3-500 m）,and in the mites from the same species of host plants that distributed in a larger area（25 km）.These populations were clustered in the same branch of the NJ phylogenetic tree and the genetic distance between them was 0-0.001.There was significant genetic differentiation of the mites collected on Q.variabilis from two different districts,Taian district of Shandong Province and Jiaozuo district of Henan Province.The mites from these two provinces were distributed in two different branches of the NJ phylogenetic tree.The population suffered long period pesticide stress had significant genetic differentiation from the population that had not experienced the pesticide stress,although they were collected from the same host species of Q.acutissima away from about 500 m.Genetic distance between the two populations was 0.015,and they were clustered in the different branch.of the NJ phylogenetic tree.Results showed that geographical isolation and pesticide stress were possibly the important factors affecting population differentiation of O.ununguis.

关 键 词：针叶小爪螨 COI 寄主植物 地理隔离 农药胁迫 种群分化 

分 类 号：S718.5[农业科学—林学]