胡萝卜幼苗根、茎、叶组织培养再生植株  

Tissue Culture and Plant Regeneration of the Root,Stem and Leaf of Carrot Seedlings

作　　者：张唯[1] 王伟[1] 续晨[1] 贲爱玲[1] 张君艳[1] 蔡小宁[1] 

机构地区：[1]南京晓庄学院,江苏南京211171

出　　处：《安徽农业科学》2012年第12期7013-7014,共2页Journal of Anhui Agricultural Sciences

基　　金：江苏省大学生实践创新训练计划项目(2011-2013);南京市重点学科建设项目(2011-2013)

摘　　要：[目的]研究胡萝卜幼苗的组织培养再生技术。[方法]以培养约2个月的胡萝卜幼苗为材料,分别取幼苗的根、茎、叶为外植体,研究不同培养基、不同外植体对愈伤组织诱导的影响,以及培养基对愈伤组织分化的影响。[结果]根、茎、叶为外植体均能产生愈伤组织,但以茎为外植体诱导的愈伤组织质量最佳,其愈伤组织的诱导率和分化率均为最高。[结论]建立了胡萝卜试管培养幼苗的愈伤组织诱导和再生植株体系,为今后的试验打下了基础。[Objective] The aim was to study plant regeneration of carrot.[Method] The root,stem and leaf of two month old seedlings were taken as explants.Effect of different medium and different explants on callus induction and callus differentiation was analyzed.[Result] The three explants could induce callus.The stem as explants was the best for callus quality,its rate of callus induction and differentiation rate were the highest.[Conclusion] The callus induction and regeneration system of carrot seedlings was established,which lays foundation for the future experiments.

关 键 词：胡萝卜 幼苗 组织培养 再生植株 

分 类 号：S631.2[农业科学—蔬菜学]