人参根际土壤微生物PCR-DGGE反应体系优化  

Optimization of PCR-DGGE Reaction System on Rhizosphere Soil Microorganism for Panax ginseng C.A.Mey.

作　　者：刘权钢[1] 金海林[1] 唐丽娜[1] 金东淳[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《安徽农业科学》2012年第12期7072-7074,7076,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选人参根际土壤微生物的PCR-DGGE反应体系。[方法]以种植1年的人参土壤为材料,利用PCR-DGGE技术,对反应体系中的几种重要参数不同梯度进行了优化研究,包括模板、dNTPs、引物及Mg2+的用量。[结果]最适宜的反应体系为:模板DNA浓度为0.8μg/μl,dNTPs浓度为0.2 mmol/L,引物浓度为0.4μmol/L,Mg2+浓度为1.5 mmol/L。[结论]该方法简便快捷,为进一步研究人参根际土壤微生物多样性奠定了基础。[Objective] To explore the PCR-DGGE reaction system on rhizosphere soil microorganism for Panax ginseng C.A.Mey..[Method] Some important factors for PCR-DGGE amplification system including the concentration of DNA template,Mg2＋,dNTPs and primers were optimized with 1-year-old P.ginseng soil as materials.[Result] An optimal system（25 μl） was obtained as 0.8 μg/μl DNA template,0.2 mmol/L dNTPs,0.4 μmol/L primer,and 1.5 mmol/L Mg2＋.[Conclusion] The method is simple and fast.This research can laid basis for further analyzing the genetic diversity of rhizosphere soil microorganism for P.ginseng.

关 键 词：人参(Panax GINSENG C.A.Mey.) 根际土壤微生物 PCR-DGGE 体系优化 

分 类 号：S561.51[农业科学—作物学]