高效液相色谱-四通道紫外可见检测法测定不同产地谷精草中槲皮素含量  

Determination of Quercetin in Eriocaulon buergerianum from Different Growing Areas using High Proficiency Liquid Chromatography Coupled with Four-channel UV-visible Detector

作　　者：陈芳[1] 冯丽娟[2] 

机构地区：[1]江汉大学化学与环境工程学院,湖北武汉430056 [2]遵义医学院珠海校区生物工程系,广东珠海519041

出　　处：《安徽农业科学》2012年第12期7091-7092,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立谷精草中槲皮素含量的测定方法,测定8个产地谷精草中槲皮素的含量。[方法]高效液相色谱-四通道紫外可见检测法,C18为色谱柱(250 mm×4.6 mm,5μm),流动相为无水甲醇-浓度0.6%磷酸(52∶48,V/V)。通过保留时间结合四波长紫外光谱定性,定性检测波长为254、360、365和370 nm。选用灵敏度最高的波长254 nm定量。[结果]槲皮素在5～30μg/ml范围内线性关系良好(R=0.999 8),平均回收率99.56%,RSD为2.30%;不同产地谷精草中槲皮素含量范围为29.5～92.5μg/g。[结论]该方法测定结果可靠,稳定易行,重复性好,可用于谷精草中槲皮素的含量测定;不同产地谷精草中槲皮素含量差异较大。[Objective] To establish a quantitative method of determination of quercetin in Eriocaulon buergerianum,and determine the content of quercetin in Eriocaulon buergerianum from different growing areas.[Method] High proficiency liquid chromatography（HPLC） coupled with four-channel UV-visible detector was used,C18 chromatographic column,mobile phase was the buffer solution of methanol and phosphoric acid（0.6%）,the volume ratio of methanol and phosphoric acid（0.6%） was 52∶48.Qualitative evaluation was done by retention time and four-channel UV spectrum at the same time,and the detection wavelength were 254,360,365,370 nm.Quantitative evaluation had done at the best sensitive wave of 254 nm.[Result] The linearity of quercetin was in the range of 5-30 μg/ml（R=0.999 8）,the average recovery was 99.56%,the RSD was 2.30%.The content of quercetin in samples from different growing areas was in the range of 29.5-92.5 μg/g.[Conclusion] The method is reliable,and can be used for quality control of Eriocaulon buergerianum.The content of quercetin in samples from different growing areas has greater difference.

关 键 词：高效液相色谱法 四通道紫外可见 谷精草 槲皮素 

分 类 号：S567[农业科学—中草药栽培] R284.1[农业科学—作物学]