春兰内生拮抗细菌gt19-1的鉴定及对辣椒胶孢炭疽菌的防效  

Identification of Antagonistic Endophyte Bacterial Strain gt19-1 from Cymbidium goeringii and Its Biocontrol Effect on Gloeosprium piperatum

作　　者：孙磊[1] 邵红[1] 刘莹[1] 徐爱芳[1] 

机构地区：[1]河北大学生命科学学院,河北省微生物多样性研究与应用实验室,河北保定071002

出　　处：《安徽农业科学》2012年第12期7145-7146,7154,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学青年基金项目(31100002);河北省自然科学基金项目(C2009000180);河北大学引进人才专项(2006-088)

摘　　要：[目的]鉴定春兰(Cymbidium goeringii)内生拮抗细菌gt19-1并研究其防效。[方法]从春兰内生细菌中筛选到一株可拮抗胶孢炭疽菌(Colletotrichum gloeosporioides Penz)的菌株gt19-1,对其进行了形态鉴定、生理生化鉴定及16S rDNA序列测定,并通过温室盆栽生防试验研究了其对辣椒炭疽菌的防治效果。[结果]菌株gt19-1为枯草芽孢杆菌(Bacillus subtilis),16S rDNA序列相似度达100%。菌株gt19-1发酵液对胶孢炭疽菌导致的辣椒炭疽病有一定抑制作用,防效达到22%。[结论]为辣椒炭疽病的生物防治奠定了基础。[Objective] The aim was to identify an antagonistic endophyte bacterial strain gt19-1 from Cymbidium goeringii and study its control effect on Gloeosprium piperatum.[Method] Endophyte bacterial strain gt19-1 with high antagonistic activity to Colletotrichum gloeosporioides Penz from Cymbidium goeringii was screened.The strain was identified by morphological observation,physiological and biochemical properties tests and 16S rDNA sequence analysis.The control effect of strain gt19-1 on Gloeosprium piperatum was studied by greenhouse biocontrol effect experiment.[Result] gt19-1 was identified as Bacillus subtilis.The 16S rDNA sequence similarity of strain gt19-1 with Bacillus subtilis was 100%.The endophyitc bacterial strain gt19-1 had biocontrol effect on pepper anthracnose,and the biocontrol efficiency was 22%.[Conclusion] The results establish the foundation for the biocontrol of the pepper anthracnose.

关 键 词：春兰 拮抗内生细菌 枯草芽孢杆菌 胶孢炭疽菌 

分 类 号：S682.31[农业科学—观赏园艺]