新型肺癌压电DNA传感器的研制  

Development of a New Lung Cancer Piezoelectric DNA Sensor

作　　者：谯康全[1] 吴永强[1] 刘新露[1] 

机构地区：[1]四川理工学院化学与制药工程学院,四川自贡643000

出　　处：《压电与声光》2012年第2期249-252,共4页Piezoelectrics & Acoustooptics

摘　　要：在压电石英金电极上自组装半胱胺膜,通过1-乙基-3-(3-甲基氨丙基)碳二亚胺盐酸盐和N-羟基琥珀酰亚胺活化的羧基化碳纳米管交联半胱胺和DNA探针,构建新的压电DNA传感器,实现对肺癌特征DNA的压电检测。该传感器检测灵敏度为27.617Hz/(μg/mL),线性范围为0.02～7.50μg/mL,再生性能好。样本分析结果与聚合酶链式反应(PCR)法无显著差异,可用于临床检测。The cysteamine film was immoboilized on a quartz crystal gold electrode＇s surface by self-assembly.DNA probes of lung cancer and cysteamine film were linked with carbonylic carbon nanotubes which been activated with 1-ethyl-3-（3-dimethyllaminopropyl） carbodiimide hydrochloride（EDC） and 1-hydroxy-2,5-pyrrolidinedione（NHS）.Thus,a new lung cancer piezoelectric DNA sensor was constructed to detect DNA labeling lung cancer,and the sensitivity was 27.617 Hz/（μg/mL）,and the linear range was from 0.02 μg/mL to 7.50 μg/mL.The DNA sensor had good regenerability,and there were not significant difference between the DNA sensor and PCR in samples detection.

关 键 词：肺癌 DNA传感器 压电检测 碳纳米管 

分 类 号：R446.90[医药卫生—诊断学] TP212.3[医药卫生—临床医学]