流产模型孕鼠脾脏调节性T细胞分离纯化方法的初步建立  

The preliminary establishment of the separation and purification methods of regulatory T cells in the spleen of abortion model rat

作　　者：殷治华[1,2] 赵富玺[1,2] 穆雅琴[2] 刘润花[2] 王健[2] 冯婷婷[1] 齐彦[1,2] 

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西大同大学免疫研究所,山西大同037009

出　　处：《中国优生与遗传杂志》2012年第3期36-37,30,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金(81170621);国家人口和计划生育委员会立项项目(C-73);山西省科学技术发展计划项目(20080311072)

摘　　要：目的探索流产模型孕鼠CD4+CD25+T细胞和CD4+CD25-T细胞快速高效的分选方法。方法采用免疫磁珠两步法分离流产模型孕鼠脾脏中T细胞,分离后的细胞经台盼蓝染色检测细胞存活率,经流式细胞仪检测分离的纯度。结果流产模型孕鼠脾脏细胞经过免疫磁珠细胞分选法2次分选得到CD4+/CD25+调节性T细胞纯度为(93.5±1.1)%,CD4+/CD25-T细胞纯度为(96.6±0.6)%。结论采用免疫磁珠细胞分选法能够快速高效的得到高纯度的CD4+CD25+T细胞和CD4+CD25-T细胞。Ojective： To explore the method of separation of CD4＋CD25＋ T cells and CD4＋ CD25-T cells in abortion model pregnant rat fast and effectively.Methods： The immune magnetic beads two-step separation is used to separate T cells of the spleen in abortion model rat,and the cell survival rate is tested by a cell to blue stain after separated,the flow cytometric tests the purity of separation.Results： The purity of CD4＋CD25＋regulatory T cells is（93.5±1.1） % in abortion model pregnant rat by magnetic beads immune cell separation method for tow times,the purity of CD4＋CD25-T cells is（96.6±0.6） %.Conclusion： The immune cells magnetic beads separation method of high speed and high efficiency can get high purity of CD4＋CD25＋ T cells and CD4＋CD25-T cells.

关 键 词：流产模型 调节性T细胞 免疫磁珠细胞分选 流式细胞仪检测 

分 类 号：R714.21[医药卫生—妇产科学]