莱菔子素对人肝细胞Ⅱ相代谢酶的作用研究  

Induction of Phase Ⅱ Metabolic Enzymes by Sulforaphene in Human Immortalized Hepatocytes

作　　者：潘萌[1] 黄睿[1] 谭洁[1,2] 李宝龙[3] 冯雪[1] 王凤前[1] 单毓娟[1,4] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081 [2]杭州市妇女医院,浙江杭州310016 [3]黑龙江中医药大学,黑龙江哈尔滨150040 [4]哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090

出　　处：《中医药信息》2012年第2期13-15,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助(81172648)

摘　　要：目的:探讨莱菔子素(sulforaphene)对人肝细胞系HHL-5细胞Ⅱ相代谢酶的影响。方法:采用噻唑蓝(MTT)比色法测定莱菔子素对HHL-5细胞的毒性作用;蛋白印记法(western blotting)检测莱菔子素对Ⅱ相代谢酶蛋白表达的影响。结果:5μmol/L莱菔子素处理HHL-5细胞24h促进细胞增殖,10～100μmol/L表现生长抑制作用;莱菔子素能明显诱导血红素加氧酶(HO-1)和硫氧还蛋白还原酶(TrxR)蛋白的表达并呈剂量依赖关系。结论:莱菔子素可诱导肝细胞Ⅱ相代谢酶HO-1和TrxR的表达,具有潜在的化学致癌物解毒功能。Objective ： To study the effect of sulforaphene on phase Ⅱ metabolic enzymes expressions in human immortalized hepatoeytes （HHL -5 cells）. Methods： MTY assay was used to test the cytotoxicity of sulfora- phene. HO - 1 and TrxR expressions were detected by western blot analysis. Results： Sulforaphene promoted HHL -5 cells growth at 5μmol/L and inhibited cells growth at 10 - 100μmoL/L. Moreover, HO - 1 and TrxR proteins were up - expressions of HO cinogen. regulated in a dose -dependent manner at 24h. Conclusion： Sulforaphene can induce the -1 and TrxR proteins ,which are attributed to the detoxification functions of chemical car-cinogen.

关 键 词：莱菔子素 莱菔硫烷 血红素加氧酶-1 硫氧还蛋白还原酶 

分 类 号：R285[医药卫生—中药学]