农杆菌介导的棉花遗传转化过程中诸因素对出愈率的影响  

Effects of some factors on callus rate in the process of cotton genetic transformation mediated by Agrobacterium tumefaciens

作　　者：孙英坤[1] 代其林[1] 龚元亚[1] 田霞[1] 谢琳[1] 杨娟[1] 王劲[1,2] 

机构地区：[1]西南科技大学植物生物技术研究中心,绵阳621010 [2]中国农业科学研究院生物技术研究所,北京100081

出　　处：《四川大学学报（自然科学版）》2012年第2期431-435,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅(09ZA034)

摘　　要：研究了农杆菌介导的棉花遗传转化过程中影响外植体出愈率的一些相关因素.结果表明:卡那霉素(Kan)的浓度以50 mg/L或75 mg/L为最好,农杆菌菌株EHA105的浸染能力较强,7 d苗龄的棉花无菌苗下胚轴为最佳的遗传转化外植体材料,下胚轴外植体经预培养后再经农杆菌菌液浸染更有利于胚性愈伤组织的形成,同时农杆菌菌液浸染时间以3 min为最佳.Some related factors influencing explants callus rate in the process of cotton genetic transformation mediated by Agrobacterium tumefaciens were studied. The results showed that the optimal concentration of Kanamycin（Kan） was 50 mg/L or 75 mg/L,the ability of disseminating of Agrobacterium tumefaciens EHA105 was more powerful,the optimal material of explant was hypocotyl of 7-daysold seedling,the preincubated hypocotyl explants before disseminated by Agrobacterium tumefaciens were more favorable to the formation of embryogenic callus,and the optimal period of disseminating was 3 minutes.

关 键 词：棉花 外植体 农杆菌 出愈率 

分 类 号：Q946[生物学—植物学]