辛伐他汀对C2C12细胞中utrophin基因表达的影响(英文)  

Effects of simvastatin on the expression of utrophin gene in C2C12 cell line

作　　者：康耀霞[1] 贾恩厚[2] 林亮[2] 段向阳[2] 侯志萍[2] 谭雪梅[1] 张义正[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]包头市疾病预防控制中心,包头市014030

出　　处：《四川大学学报（自然科学版）》2012年第2期448-452,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家科技支撑项目(2007BAD78B03)

摘　　要：本文采用MTT法,观察不同浓度辛伐他汀(0,1,3,10,30 and 100μmol/L)对C2C12细胞活力的影响,并通过定量RT-PCR方法对utrophin和TNF-α基因的mRNA的表达进行了研究.随着辛伐他汀浓度和作用时间的增加,C2C12细胞的活力在降低;不同浓度辛伐他汀对utrophin和TNF-α的mRNA表达具有影响作用,为阐明辛伐他汀的肌毒性作用机理提供了新的思路.The effects of simvastatin on the cell viability and the mRNA expressed state of utrophin gene in C2C12 cells were measured. Simultaneously, the mRNA of tumor necrosis factor a （TNF-a） was examined to get the information of inflammatory cytokines after simvastatin treated C2C12 cells. C2C12 cells were treated by 0, 1, 3, 10, 30 and 100/μmol/L simvastatin for 24 h and 48 h. MTT [3-（4, 5-dim- ethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide] assay was used to test cell viability. The real-time quantitative RT-PCR was used to test mRNA expression. Simvastatin inhibits the cell growth with the concentration increase and effect mRNA levels of utrophin and TNF-a in C2C12 cell line. These results might be helpful to elucidate the mechanism of side effects occur in skeletal muscle induced by simvasta- tin.

关 键 词：辛伐他汀 UTROPHIN C2C12细胞 

分 类 号：Q23[生物学—细胞生物学] R322.4[医药卫生—人体解剖和组织胚胎学]