湿地松抗病种子园的遗传多样性分析  

GENETIC VARIATION OF RAPD MARKERS IN A DISEASE RESISTANT SEED ORCHARD OF \%PINUS ELLIOTTII\% ENGELM

作　　者：易能君[1] 韩正敏[1] 尹佟明[1] 黄敏仁[1] 曹汉洋 

机构地区：[1]南京林业大学林木遗传与基因工程国家林业局重点实验室,南京210037 [2]福建沙县官庄林场,沙县366500

出　　处：《林业科学》2000年第z01期51-55,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金!资助项目 ( 3 9770 62 8)

摘　　要：本文利用RAPD分子标记从DNA水平上研究了湿地松 (PinuselliottiiEngelm)种子园的遗传多样性和连锁不平衡状况。结果表明 ,有效等位基因数目在各位点间变动范围为 1 0 47～ 1 996 ,平均为 1 4 499;基因多样度范围为 0 0 44 9～ 0 4 99,平均为 0 2 781 ;Shannon信息指数的范围为 0 1 0 95～ 0 6 92 2 ,平均为 0 4 32 7;这3个遗传多样性度量的大小顺序基本相同。 1 0 4个分离位点共有 5 35 6个位点对 ,其中 35 5对在显著性水平为0 0 5下达到显著的连锁不平衡状态。Genetic variation and linkage disequilibrium in a disease resistant seed orchard of \%Pinus elliottii\% Engelm were investigated by using RAPD markers in this paper. The results showed that the effective number of alleles per locus ranged between 1.047 and 1.996, averaging 1.449; Estimates of gene diversity per locus ranged between 0.0449 and 0.499, averaging 0.2781;and the Shannon's information index ranged between 0.1095 and 0.6922, averaging 0.4327. The order of these three measures per locus was the same. Out of 5356 pairs of RAPD loci in 104 segragating loci, 355 pairs showed significant deviations from linkage equilibrium. These results indicated that high genetic variation and linkage disequilibrium were harbored in this seed orchard.

关 键 词：温地松 种子园 RAPD 遗传多样性 连锁不平衡 

分 类 号：S791.246[农业科学—林木遗传育种] S722.83[农业科学—林学]