不同强度跑台运动对大鼠学习记忆能力及海马CA3区突触超微结构的影响  

Effect of different Intensity Exercises in Treadmill on Learning and Memory and The Synaptic Ultrastructure in Hippocampal CA3 Area in Rats

作　　者：龚云[1] 

机构地区：[1]西北师范大学体育学院,甘肃兰州730700

出　　处：《北京体育大学学报》2012年第3期63-66,共4页Journal of Beijing Sport University

基　　金：西北师范大学青年教师科研能力提升计划项目(编号:SKQNYB10033)

摘　　要：探讨不同强度跑台运动对大鼠学习记忆能力及海马CA3区超微结构的影响。40只Wister大鼠随机被分为4组(对照组、低强度运动组、中强度运动组、高强度运动组,各10只),按各自强度运动60 d后,通过一次性被动回避反应实验,逐只测量其步入潜伏期的时间(STL);后每组任选5只大鼠,取右侧海马CA3区按常规方法制作超薄切片,60K倍透射电镜观察并拍照,Motic Images Advanced 3.1软件测量相关突触界面结构。结果表明:低强度运动组大鼠,电击后24 h步入潜伏期的时间显著延长(P<0.05),海马CA3区突触后膜致密物质非常显著增厚(P<0.01)。突触界面呈现平直型、正向弯曲型和负向弯曲型三种,以平直型为主。由此可见,低等强度运动可增强记忆能力,并影响海马CA3区神经元突触结构发生可塑性变化,突触后膜致密物质厚度与记忆能力呈正相关。Effect of the different intensity treadmill exercise on learning and memory ability and hippocampal CA3 area ultrastructure was studied in rats.40 SD rats were randomly divided into four groups（control group,low-intensity exercise group,medium-intensity exercise group and high-intensity exercise group）,after 60d exercise according to their respective intensity,through the one-trial passive avoidance response,the time to step-through lateney（STL） of rats was measured in every exercise group.Then 5 rats were chosen from every group,through normal ultrathin section made method,hippocampal CA3 area was observed under JEM-1230EX transmission electron microscope and photographed.The results showed that： 24 hours of STL period after electrical shock was significantly longer（P0.05） in low-intensity exercise group rats,and the hippocampal CA3 area postsynaptic density material became significantly thicker（P0.01）.The curved type of synaptic interface could be divided into positively curved,negatively curved and flat,the flat type was the main.As a result,low-intensity exercise can increase memory capacity,and make the neuron synapse occurring plastic changes in the hippocampal CA3 area.The thickness of postsynaptic density material came into being a positive correlation with the strength of memory.

关 键 词：学习记忆 突触 海马CA3区 超微结构 跑台运动 

分 类 号：G804.2[文化科学—运动人体科学] R87[文化科学—体育学]