Investigation of Breaking Efficiency of N-Glycosidic Bond Between Sulfonamide and Honey  

作　　者：WANG Yan-hua LI Li CHEN Chia-hung CHENG Shuenn-ren LEE Mu-sheng YU Pu-ping CHANG-CHIEN Guo-ping 

机构地区：[1]College of Biology and Enviornmental Engineering, Zhejiang Shuren University, Hangzhou 310015, P. R. China [2]Super Micro Mass Research ＆ Technology Center [3]Department of Business Administration [4]Department of Mechanical Engineering, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China [5]Environmental Protection Bureau Kaohsiung City Government, Keelung 20143, Taiwan, P. R. China

出　　处：《Chemical Research in Chinese Universities》2012年第2期195-199,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20907046);the Natural Science Foundation of Zhejiang Province of China(No.Y4100623)

摘　　要：Sulfonamide residue in honey existed in a form bonded to sugar via the N-glycosidic bond.It would result in the possible underestimation of concentration of sulfonamide if it is not decomposed by chemical methods.However,in China's Mainland and Taiwan（P.R.China）,the regulation for sulfonamide residue analysis does not include hydrolysis and has been applied to a very broad range of samples,for example,egg,milk,meat,seafood as well as honey.This paper demonstrates the necessity of hydrolysis of it prior to extracting honey.The breaking efficiencies of N-glycosidic bond were investigated by 2 mol/L hydrochloric acid,pure methanol and 0.5 mol/L hydrochloric acid in methanol,respectively.It was found that acid plays the key role in breaking the N-glycosidic bond,and it was also noticed that the dissolution of liberated sulfonamide in methanol could carry the reaction forward in favor of breaking the N-glycosidic bond.Sulfonamide residue in honey existed in a form bonded to sugar via the N-glycosidic bond.It would result in the possible underestimation of concentration of sulfonamide if it is not decomposed by chemical methods.However,in China's Mainland and Taiwan（P.R.China）,the regulation for sulfonamide residue analysis does not include hydrolysis and has been applied to a very broad range of samples,for example,egg,milk,meat,seafood as well as honey.This paper demonstrates the necessity of hydrolysis of it prior to extracting honey.The breaking efficiencies of N-glycosidic bond were investigated by 2 mol/L hydrochloric acid,pure methanol and 0.5 mol/L hydrochloric acid in methanol,respectively.It was found that acid plays the key role in breaking the N-glycosidic bond,and it was also noticed that the dissolution of liberated sulfonamide in methanol could carry the reaction forward in favor of breaking the N-glycosidic bond.

关 键 词：SULFONAMIDE HONEY Liquid chromatography-electrospray ionization tandem mass spectrometry（LC-ESIMS/MS） N-Glycosidic bond 

分 类 号：S858.31[农业科学—临床兽医学] TS264.3[农业科学—兽医学]