Anticancer Effects of Fusion Protein CAtin on DMBA-induced Carcinogenesis in Buccal Pouch of Chinese Hamster  

作　　者：BAI Jie-ying LI Xiao LI Chang ZHANG Xiao-fei LI Zhi-xin ZHAO Shuang LIU Xiao ZENG Lin CHI Bao-rong 

机构地区：[1]Laboratory Animal Center,Academy of Military Medical Sciences,Beijing 100071,P.R.China [2]Department of Gastroenterology,the First Hospital of Jilin University,Changehun 130021,P.R.China [3]Institute of Radiation Medicine,Academy of Military Medical Sciences,Beoing 100071,P.R.China [4]Department of Histology and Embryology,Jilin Medical College,Jilin 132001,P.R.China

出　　处：《Chemical Research in Chinese Universities》2012年第2期269-275,共7页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.30870347,81101140);the Youth Foundation of Laboratory Animal Center of Academy of Military Medical Sciences of China(No.QN2010-001);the National Science Foundation for Post-doctoral Scientits of China(No.20100481057)

摘　　要：Aberrant expression of carcinoembryonic antigen（CEA） is a common feature for multiple types of cancer,which makes it an attractive target for anticancer therapy.CAtin is a novel dual cancer-specific fusion protein,composed of an anti-CEA single-chain disulfide-stabilized Fv antibody（scdsFv） and Apoptin,a tumor-specific apoptosis-inducing protein.Oral squamous cell carcinoma（OSCC） is an important healthcare problem in the clinic.To evaluate the anticancer effects of CAtin on OSCC,7,12-dimethylbenz[a]anthracene（DMBA） was used to induce oral carcinogenesis and premalignant lesions in the buccal pouch of Chinese hamster,and the antitumor effects of CAtin were determined in pre-cancer,cancer and post-operatative cancer models,respectively.The results show that the administration of CAtin delayed the malignant transformation of early stage cancerous lesions,inhibited the growth of established solid oral tumors and reduced the post-operatative relapse of lesions,with no significant systemic toxicity.This study demonstrates that CAtin may have potential for the treatment of OSCC,and the development of preventive strategies based on CAtin may offer a practical approach for the treatment of human oral tumors.Aberrant expression of carcinoembryonic antigen（CEA） is a common feature for multiple types of cancer,which makes it an attractive target for anticancer therapy.CAtin is a novel dual cancer-specific fusion protein,composed of an anti-CEA single-chain disulfide-stabilized Fv antibody（scdsFv） and Apoptin,a tumor-specific apoptosis-inducing protein.Oral squamous cell carcinoma（OSCC） is an important healthcare problem in the clinic.To evaluate the anticancer effects of CAtin on OSCC,7,12-dimethylbenz[a]anthracene（DMBA） was used to induce oral carcinogenesis and premalignant lesions in the buccal pouch of Chinese hamster,and the antitumor effects of CAtin were determined in pre-cancer,cancer and post-operatative cancer models,respectively.The results show that the administration of CAtin delayed the malignant transformation of early stage cancerous lesions,inhibited the growth of established solid oral tumors and reduced the post-operatative relapse of lesions,with no significant systemic toxicity.This study demonstrates that CAtin may have potential for the treatment of OSCC,and the development of preventive strategies based on CAtin may offer a practical approach for the treatment of human oral tumors.

关 键 词：7 12-Dimethylbenz[a]anthracene（DMBA） Anti-tumor CAtin Oral squamous cell carcinoma（OSCC） Hamster cheek pouch 

分 类 号：Q78[生物学—分子生物学] S896.6[农业科学—特种经济动物饲养]