缬沙坦对甲亢性心脏病大鼠心肌组织TGF-β1、Smad3和Smad7表达的影响  

Effects of valsartan on expressions of TGF-β1,Smad3 and Smad7 in ventricular tissue of rats with hyperthyroidism cardiopathy

作　　者：李拜红[1] 金伟东[1] 王晓彦[1] 陶以嘉[1] 施晶晶[1] 陈翀昊[1] 魏哲强[2] 朱栋晓[3] 

机构地区：[1]南通大学第三附属医院心内科,无锡214000 [2]南通大学第三附属医院病理科,无锡214000 [3]南通大学第三附属医院超声室,无锡214000

出　　处：《郑州大学学报（医学版）》2012年第2期178-182,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：无锡市医院管理中心医学科技发展基金资助项目

摘　　要：目的:探讨缬沙坦对甲亢性心脏病大鼠左室心肌组织中TGF-β1、Smad3和Smad7表达的影响。方法:SD大鼠40只,随机分为对照组、模型组、缬沙坦小剂量组(VL组)和缬沙坦大剂量组(VH组)。模型组、VL和VH组予L-甲状腺素0.5mg/(kg.d)腹腔注射28d,VL组和VH组在建模同时灌胃缬沙坦10和30mg/(kg.d)。造模前后分别采取静脉血,用化学发光法检测血清TT3、TT4水平;第28天行超声心动图检测左室舒张末期内径(LVDD)、左室后壁(LVPW)厚度、室间隔(IVS)厚度;计算心肌肥厚指标;HE染色和Masson染色观察左室心肌组织形态结构,测定左心室肌细胞直径和胶原容积分数,免疫组化法观察TGF-β1、Smad3及Smad7的表达。结果:与对照组相比,模型组大鼠左室腔扩大,室壁增厚,左心指数、心肌细胞直径、胶原容积分数增大;左心室心肌组织TGF-β1、Smad3表达明显增强,Smad7表达略有增加。VH和VL组心肌重构指标均较模型组改善,心肌组织TGF-β1、Smad3表达下降,而Smad7表达明显上升;VH和VL组间各指标差异无统计学意义(P>0.05)。结论:缬沙坦可通过调节TGF-β1、Smad3及Smad7的表达,抑制心肌重构,保护心功能。Aim：To investigate the effects of valsartan on expressions of TGF-β1,Smad3,and Smad7 in ventricular tissue of rats induced by levothyroxine（L-thy）.Methods：A rat model of hyperthyroidism was established by daily intraperitoneal injections of L-thy 0.5 mg/（kg·d） for 28 days.Rats were randomly divided into four groups and 10 in each group：control group,model group,VL group（L-thy＋10 mg/kg valsartan） and VH group（L-thy＋30 mg/kg valsartan）.At the end of the 4 week treatment,all rats underwent echocardiography,and LVDD,IVS and LVPW thickness were measured.Ventricular tissue was then collected to detect cardiac hypertrophy index,and cardiomyocyte diameter.Structural changes were obtained.Proteins of TGF-β1,Smad3,and Smad7 in left ventricle were localized by immunohistochemistry.Results：Compared with control group,rats in model group displayed myocardial hypertrophy and morphological changes,collagen content was significantly higher,and the expressions of TGF-β1 and Smad3 increased（P0.01）.Compared with model group,LVDD,thickness of IVS and LVPW,the cardiac hypertrophy index,the average diameter of myocardium cell,collagen volume fraction and the expressions of TGF-β1 and Smad3 in VL and VH groups were improved significantly（P0.01）,and Smad7 obviously increased（P0.01）.There were no significant differences in the indexes mentioned above between VH and VL group（P0.05）.Conclusion：Valsartan can effectively inhibit the remodeling of ventricle,ameliorate the hypertrophic states of myocardium induced by L-thy through regulating the expressions of TGF-β1,Smad3 and Smad7.

关 键 词：大鼠 甲状腺功能亢进 心脏病 缬沙坦 TGF-Β1 SMAD3 SMAD7 

分 类 号：R514.85[医药卫生—内科学]