甘蓝型油菜分子标记在重组自交系群体中的偏分离分析  

Segregation Distortion Analysis of Molecular Markers in RIL Population of Brassica napus

作　　者：丁广大[1,2] 杨美[1,2] 李兴美[3] 石磊[1,2] 徐芳森[1,2] 

机构地区：[1]华中农业大学微量元素研究中心,武汉430070 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070 [3]汉口学院,武汉430212

出　　处：《中国农业科技导报》2012年第2期56-61,共6页Journal of Agricultural Science and Technology

基　　金：国家973计划项目(2011CB100301);国家863计划项目(2006AA10A112)资助

摘　　要：以甘蓝型油菜磷高效品种鄂油长荚和磷低效品种B104-2为亲本杂交得到的F10群体作为构图群体,共124个单株,构建了一张含733个分子标记的甘蓝型油菜分子遗传图谱。在这些分子标记中,通过偏分离分析发现,有218个分子标记发生奇异分离(P<0.05),占总连图标记数的29.7%。其中,偏向母本B104-2有136个,占62.4%,偏向父本鄂油长荚有82个,占37.6%。同时,在13个连锁群上发现了24个偏分离热点区域。对这种偏分离现象和原因进行了分析讨论。A genetic linkage map comprising 733 molecular markers of Brassica napus was constructed using F10 population consisting of 124 recombinant inbred lines derived from a cross between P-inefficient cv.B104-2 and P-efficient cv.Eyouchangjia.Segregation distortion analysis was performed among the 733 polymorphic markers.The result indicated that 218 markers(29.7%) showed significant segregation distortion(P<0.05),favoring either the marker alleles of female parent B104-2(136,62.4%) or male parent Eyouchangjia(82,37.6%).Totally,24 segregation distortion regions(SDRs) were detected among 13 different chromosomes.Based on this result,the phenomenon and causes for the segregation distortion were analyzed and discussed in this paper.

关 键 词：甘蓝型油菜 重组自交系 分子标记 偏分离 

分 类 号：S634.3[农业科学—蔬菜学]