放射免疫沉淀试验鉴定单纯疱疹病毒糖蛋白C  

Identification of glycoprotein C of herpes simplex virus

作　　者：喻启桂[1] 秦克锋[1] 高谦[1] 汪美先[1] 姜绍谆[1] 王海涛[1] 

机构地区：[1]第四军医大学微生物学教研室,西安710033

出　　处：《中国免疫学杂志》1990年第6期333-337,共5页Chinese Journal of Immunology

摘　　要：用放射免疫沉淀试验和单纯疱疹病毒1型、2型(HSV—1/HSV-2)型间重组株作物理谱图的方法.鉴定出抗HSV的单克隆抗体(McAb) 1A12、Mad-2、2D11、CM-D3、2A8和1C_4的靶抗原是一组分子量在105～130Ka之间的糖蛋白,其编码基因定位于长单一序列(UL)上,在0.536～0.682遗传单位之间,与gC的编码基因部分重叠。故认为这些McAbs的靶抗原为gC,其中,Mad-2和CM-D3分别为HSV-1和HSV-2型特异性,其靶抗原分别为gC-1和gC-2。ELISA阻断试验结果表明,4株型共同性McAbs 1A12、2D11、2A8和1C4.抗gC上4个独立的抗原位点。A group of glycoproteins with ⅥW 105- 130 K D from BHK-21 cell infected with Herpes Simplex Virus (HSV) were precipitated by six monoclonal antibodies (McAb). 1 A 12. Mad-2, 2D11, CM-D3, 2A8 and lC4,by using immunoprecipate technique. Im munoassays of BHK-21 cells infected with HSV-1/HSV-2 intertypic recom binants localized the genes encoding the target antigens of 1 A12, Mad-2, 2Dll, CM-D3, 2A8 and 1 C4 to the unique long region of HSV genesome at mapping unit 0.536—0.682 overlapping the gene encoding glycoprotein C(gC). De pending on reactivity with HSV- 1 and HSV-2 infected BHK-21 cells, Mad-2 shows HSV type-1 specificity, C M-D 3 shows HSV ty pe-2 specificity and the other four McAbs show type-common spe. cifity. So Mad-2 is against gC-1, CM D3 is against gC - 2 and 1 A12, 2 A8, 2 D11, 1 C4 are against type-common gC. We labelled the four McAbs directed at type-common gC with horseradise peroxidase (HRP)by the modified method of Nakane. ELISA blocking test was done to test the relationships of them. The result is that those four McAbs are against four distinct antigenic sites on type-common gC.

关 键 词：单纯疱疹病毒 放射免疫 沉淀试验 

分 类 号：R392－33[医药卫生—免疫学]