透射电镜样品制备中常见污染问题的探讨  

The discussion of common pollution in the sample preparation of transmission electron microscopy

作　　者：于红丽[1] 董富兴[1] 戚大石[2] 熊晔[2] 董红燕[1] 

机构地区：[1]徐州医学院神经生物学研究中心,江苏徐州221002 [2]徐州医学院神经生物学教研室

出　　处：《徐州医学院学报》2012年第2期92-94,共3页Acta Academiae Medicinae Xuzhou

基　　金：徐州医学院科研课题（2010KJ18）;徐州医学院院长专项人才基金（2010KJZ11）

摘　　要：透射电镜生物样品制备最常用的是超薄切片法,超薄切片技术的制片过程很复杂,要求很细致,稍有不慎便会造成污染,影响图片质量.本文就如何减少样品制备污染问题,总结了本实验室长期以来的制样经验,供广大电镜工作者,特别是初学者借鉴.The most common technology in the biological sample preparation of transmission electron microscopy is ultramicrotomy. The production of ultramicrotomy is sophisticated, and the demanding is careful, even a slightest mistake can lead to pollution and affect image quality. This paper aims at how to reduce pollution in sample preparation, summarizes the experience of sample preparation in our laboratory for the majority of the electron microscopy workers, especially the beginners to learn.

关 键 词：透射电镜 制样 污染 

分 类 号：Q-336[生物学]