单核细胞趋化蛋白-1通过促进胰淀素表达调控胰岛β细胞凋亡  

Monocyte chemoattractant protein-1 regulates apoptosis of islet β-cells by promoting expression of amylin

作　　者：朱晗玉[1] 洪权[1] 张冬[1] 耿文佳[1] 刘沫言[1] 谢院生[1] 陈香美[1] 

机构地区：[1]解放军总医院肾病科全军肾脏病中心暨国家重点实验室,北京100853

出　　处：《军医进修学院学报》2012年第5期489-491,499,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(61101218;81102673);国家973项目(2007CB507400);全军医学科研"十一五"专项基金(08Z034)~~

摘　　要：目的观察单核细胞趋化蛋白-1(MCP-1)对胰岛β细胞凋亡的影响,并探讨其可能机制。方法体外培养大鼠胰岛β细胞株INS-1E,给予1ng/ml的MCP-1刺激48h后,real-time PCR及western blot检测细胞中Amylin的mRNA及蛋白表达水平,同时流式细胞仪检测细胞凋亡情况。细胞转染Amylin SiRNA,再给予MCP-1刺激,同样采用流式细胞仪技术检测细胞凋亡情况。结果 INS-1E细胞经MCP-1刺激后,细胞中Amylin的mRNA及蛋白表达水平均升高(P<0.05),细胞出现了凋亡现象。而预先转染了抑制Amylin表达的siRNA后再进行MCP-1的刺激,此时细胞的凋亡率低于单独刺激组(P<0.05)。结论 MCP-1通过促进胰淀素的表达调控胰岛β细胞的凋亡,从而影响血糖代谢。Objective To observe the effect of monocyte chemoattractant protein-l（MCP-1） on apoptosis of pancreatic β-cells and study its possible mechanism. Methods Forty-eight hours after in vitro cultured rat pancreatic β cell line INS-1E was stimulated with 1ng/ml MCP-1, expression levels of amylin mRNA and protein in rat pancreatic β cells were measured by real-time PCR and Western blot, respectively. Apoptosis of rat pancreatic β cells was detected by flow cytometry. Forty-eight hours after in vitro cultured rat pancreatic β cells transfected with amylin SiRNA were stimulated again with lng/ml MCP-1, their apoptosis was detected by flow cytometry. Results The expression levels of amylin mRNA and protein in INS-1E cells were significantly higher after MCP-1 stimulation than before MCP-1 stimulation（P〈0.05） and the apoptosis of INS-1E cells occurred. However, the apoptosis rate of INS-1E cells was lower when the expression level of amylin mRNA in INS-1E cells was stimulated with MCP-1 and transfected with amylin mRNA than when the expression level of amylin mRNA in INS-1E cells was only stimulated with MCP-1 （P〈0.05）. Conclusion MCP-1 can regulate the apoptosis of rat pancreatic β ceils by promoting the expression of amylin, thus influencing the metabolism blood sugar.

关 键 词：单核细胞趋化蛋白1 胰淀素 胰岛Β细胞 凋亡 

分 类 号：R347.8[医药卫生—基础医学]