激素性股骨头坏死模型骨髓间充质干细胞的生物学特性评估  

Biological characteristics of mesenchymal stem cells in hormone-induced rat femoral head necrosis model

作　　者：李志锐[1] 王玉[2] 陈继凤[2] 赵斌[2] 彭江[2] 张莉[2] 卢世璧[2] 董纪元[1] 

机构地区：[1]解放军总医院骨科四病区,北京100853 [2]解放军总医院骨科研究所,北京100853

出　　处：《军医进修学院学报》2012年第5期525-527,552,共4页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的评估激素性股骨头坏死模型自体骨髓间充质干细胞增值能力及定向诱导能力。方法建立SD大鼠激素性股骨头坏死动物模型,行病理检查,验证模型。取模型组和对照组的骨髓间充质干细胞进行细胞生物学特性评估。结果联合运用牛血清和激素能够较好地建立激素性鼠股骨头缺血性坏死动物模型,激素性股骨头坏死SD大鼠的骨髓间充质干细胞,增值速率显著慢于对照组(P<0.05),其骨髓间充质干细胞向成骨细胞定向诱导后ALP染色阳性,但茜素红染色显示钙结节数量少于对照组,向成脂细胞定向诱后油红O染色示脂肪细胞少于对照组。结论实验组骨髓间充质干细胞仍具备干细胞的生物学特性,但相对于对照组其增殖能力减弱,成骨、成脂潜能减弱。Objective To assess the proliferation and directional induction ability of mesenchymal stem cells in hormone-induced rat femoral head necrosis model. Methods A SD rat femoral head necrosis model was induced with hormone and bovine serum to observe and verify its pathological change. Bone marrow mesenchymal stem cells were taken from model group and control group to assess their biological characteristics. Results The bovine serum and hormone-induced rat femoral head necrosis model was successfully established. The proliferation rate of bone marrow mesenchymal stem cells was significantly lower in model group than in control group（P〈0.05）. The positive staining of bone marrow mesenchymal stem cells was observed after directional induction with hormone or bovine serum. However, alizarin red and oil red O staining showed that the number of calcium nodules and lipoblasts was significantly less in model group than in control group after directional induction with hormone or bovine serum. Conclusion The bone marrow mesenchymal stem cells in model group still show their biological characteristics after directional induction, but their proliferation, bone and adipose formation potentials are weaker in model group than in control group.

关 键 词：激素 股骨头坏死 动物模型 鼠 骨髓间充质干细胞 

分 类 号：R34[医药卫生—基础医学]