KISS-1和GPR54基因在雌性性早熟大鼠下丘脑中的表达  

Expressions of hypothalamic KISS-1 and G protein-couple receptor 54 genes in female rats with precocious puberty

作　　者：葛伟[1] 王海莲[2] 薛江[2] 

机构地区：[1]山东大学齐鲁医院儿科,济南250012 [2]山东大学第二医院儿科,济南250033

出　　处：《山东大学学报（医学版）》2012年第4期71-75,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2007C137)

摘　　要：目的研究KISS-1和GPR54基因在雌性性早熟大鼠下丘脑中的表达,探讨其在性早熟发生中的作用。方法将雌性26日龄SD大鼠50只随机分为实验1组、实验2组、对照1组、对照2组、对照3组。实验组皮下注射N-甲基-D,L-天冬氨酸(NMA)每天2次直至阴道开放,对照组注射生理盐水,观察大鼠阴道开放时间及性周期,测量子宫指数、卵巢指数、卵巢黄体出现率、子宫壁厚度、黄体生成素(LH)浓度。采用Real-Time RT-PCR方法,检测KISS-1和GPR54基因在雌性性早熟大鼠下丘脑中的表达。结果实验组大鼠阴道开放及首次发情间期出现的时间较对照组提前(P<0.05)。随着青春期发育,各项观测指标均逐渐升高(P<0.05),在相同的性发育阶段,实验组和对照组各项观测指标无明显差异(P>0.05)。结论 KISS-1和GPR54基因的表达与性发育阶段密切相关,提示KISS-1与GPR54基因在真性性早熟的启动和发展过程中起重要作用。Objective To investigate expressions of hypothalamic KISS-1 and G protein-couple receptor 54（GPR54） genes in female rats with precocious puberty and their effects on development of precocious puberty.Methods Fifty 26-day-old female rats were randomly divided into 5 groups,the first experimental group,the second experimental group,the first control group,the second control group,and the third control group.Experimental groups received a subcutaneous injection of N-methyl-DL-aspartate acid（NMA） twice a day until vaginal opening（VO） occurred,while control groups received normal saline.Parameters of puberty were analyzed,including the day of VO,estrous cycle of the rat,index of the ovary,index of the uterus,incidence of the corpora lutea,thickness of the uterus and serum luteinizing hormone（LH）.Expressions of hypothalamic KISS-1 and GPR54 genes in the five groups were detected by Real-time reverse transcription polymerase chain reaction（Real-time RT-PCR）.Results The age of VO and first diestrus in experimental groups were earlier than in control groups（P0.05）.All parameters of puberty gradually increased with the development of puberty（P0.05）.In the same puberty developmental stage,parameters in experimental groups were not different from those in control groups（P0.05）.Conclusion Expressions of KISS-1 and GPR54 genes are associated with developmental stages of puberty,indicating that KISS-1 and GPR54 genes may play important roles in onset and development of true precocious puberty.

关 键 词：青春期 性早熟 基因 KISS-1 基因 GPR54 

分 类 号：R725.8[医药卫生—儿科]