干扰隐花色素Cry1a与Cry1b基因的表达对水稻若干农艺性状的影响  

Effects of interfering the expression of cryptochrome Cry1a/Cry1b genes on several rice agronomic traits

作　　者：李毓[1,2] 庄伟建[1,3] 庄春红 王乃元[2] 戴飞 

机构地区：[1]福建农林大学作物科学学院,福建福州350002 [2]福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州350002 [3]福建省分子与细胞生物学重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2012年第2期153-158,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技计划重点项目(2008I01021117);国家高技术研究发展计划(2007AA10Z181)资助项目

摘　　要：利用已公布的水稻Cry1a与Cry1b基因序列设计引物,PCR扩增基因部分片段,构建了一个包含2个隐花色素基因片段的ihpRNA表达载体pSC1301-347-Cry1aCry1b,并通过农杆菌介导转化水稻,以期同时导致这2个基因功能缺失.根据转基因植株主要农艺性状的表现,初步分析了Cry1a与Cry1b对水稻重要农艺性状的影响.结果表明,转基因水稻开花期推迟16 d,株高增加明显,粒型显著变长,而其他所观察的性状无明显改变.According to the published rice Cry1a and Cryl b gene sequences, we designed the related primers to get part of the gene fragments by PCR and constructed an ihpRNA plant expression vector named pSC1301-347-Cry1aCry1b, then introduced it into rice by Agrobacter/um-medlated genetic transformation so as to cause the functional loss of the two genes at the same time. The effects of rice Cry1a and Cry1b on important agronomic traits were preliminarily analyzed based on the performance of the transformants. The results indicated that the transgenic plants retarded flowering over 16 d; the plant height increased significantly with the grain shape evidently becoming longer while the other traits observed did not change apparently.

关 键 词：水稻 隐花色素 Cry1a/Cry1b基因 ihpRNA 农艺性状 

分 类 号：Q943.1[生物学—植物学] S511.035.3[农业科学—作物学]