过量表达拟南芥高亲和性磷酸载体蛋白基因提高转基因烟草对磷的吸收(英文)  

Overexpression of an Arabidopsis thaliana High-Affinity Phosphate Transporter Gene to Enhance Uptake of Phosphorus in Transgenic Tobacco Plants

作　　者：陈奇[1] 赵玥[1] 张亚楠[1] 郝永生[1] 武孔焕[1] 李昆志[1] 玉永雄[2] 陈丽梅[1] 

机构地区：[1]昆明理工大学呈贡校区生物工程技术研究中心,云南昆明650500 [2]西南大学动物科学与技术学院,重庆400715

出　　处：《昆明理工大学学报（自然科学版）》2012年第1期61-66,共6页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家973项目(项目编号:2007CB108901);昆明理工大学优秀博士培育基金项目(项目编号:41118010)

摘　　要：磷是所有生物生长必需的大量营养元素.酸性土壤占世界可耕地面积30%以上,在酸性土壤中,大部分磷以不溶性形式存在,很难被植物吸收和利用.为了增加植物对酸性土壤不溶性磷的吸收能力,本研究利用CaMV35S启动子在转基因烟草中过量表达拟南芥高亲和性磷酸载体蛋白(AtPTI)基因.研究结果表明在酸性土壤中生长时,转基因烟草叶片磷的含量及其生物量均高于不转基因的野生型(WT)植物(对照).转基因植物可以正常生长、开花和结实,而WT植物的开花时间提前且不能正常结果.这些结果证实在植物中过量表达AtPTI基因是提高植物吸收酸性土壤中磷及其产量的一个有效策略.Phosphorus（P） is one of the major nutrients required for all living organisms.Acidic soils cover more than 30% of the world  s arable land.However,most P presents as insoluble form in acidic soils,which is difficult to utilize by plants.Thereby,P deficiency is a major limiting factor for plant growth and crop production in acidic soils.In order to enhance the ability of plants to utilize the sparingly soluble P in acidic soils,a high-affinity P transporter gene from Arabidopsis thaliana（AtPTI） is overexpressed in transgenic tobacco plants under the control of CaMV35S promoter.Further analyses show that the P content in the transgenic tobacco leaves is higher than that in untransformed wild type（WT） plants（control） when grown under acid soil conditions.Furthermore,the biomass of the transgenic plants is also higher than that of the control plants.The transgenic plants normally flower and produce fruits whereas the WT plants flower earlier than the transgenic lines and fail to produce fruits.The results demonstrate that overexpression of the AtPTI gene is an effective strategy to enhance the plant P uptake ability and their biomass production in acidic soils.

关 键 词：磷吸收 转基因烟草 酸性土壤 高亲和性磷酸转运载体 过量表达 

分 类 号：Q344.13[生物学—遗传学]