三种不同方法诱导3T3-L1脂肪细胞胰岛素抵抗的对比研究  

Comparative Study of Inducing Insulin Resistance in 3T3-L1 Adipocytes with Three Different Methods

作　　者：陈立[1] 杨明炜[2] 库宝庆[1] 莫阔[1] 汪继敏[1] 王晓翠[1] 马聪[1] 

机构地区：[1]湖北省襄阳市中心医院,441021 [2]华中科技大学同济医学院中西医结合研究所

出　　处：《中国全科医学》2012年第9期999-1001,共3页Chinese General Practice

基　　金：湖北省卫生厅2004年度中医药中西结合课题(2010.5-2011.5)

摘　　要：目的对比地塞米松、游离脂肪酸、高糖高胰岛素3种不同方法诱导的胰岛素抵抗3T3-L1脂肪细胞的葡萄糖消耗量及葡萄糖转运子4(Glut4)的表达。方法采用3种不同方法诱导3T3-L1脂肪细胞产生胰岛素抵抗,分别检测各组细胞12、24、36、48、60 h时的葡萄糖消耗量及Glut4蛋白的表达。结果在24 h内,地塞米松组、游离脂肪酸组、高糖高胰岛素组细胞的葡萄糖消耗量与正常对照组比较均显著降低,差异有统计学意义(P<0.05);在24 h以后,游离脂肪酸组葡萄糖消耗量与正常对照组比较差异无统计学意义(P>0.05);而地塞米松组葡萄糖消耗量相较正常对照组仍显著降低,高糖高胰岛素组葡萄糖消耗量较地塞米松组显著降低,差异有统计学意义(P<0.05)。地塞米松组及游离脂肪酸组的Glut4表达水平较正常对照组显著降低,差异有统计学意义(P<0.01);相对于地塞米松组及游离脂肪酸组,高糖高胰岛素组的Glut4表达水平显著降低,差异有统计学意义(P<0.01)。结论高糖高胰岛素能够诱导3T3-L1脂肪细胞产生更强的胰岛素抵抗,这种抗性的产生可能是因为抑制了Glut4蛋白的表达获得的。Objective To observe the glucose consumption and Glut4 expression in inducing insulin resistance in 3T3 - L1 adipocytes with three methods （dexamethasone, free fatty acids, high glucose and hyperinsulinism） . Methods Three methods were used to induce insulin resistance in 3T3 -L1 adipocytes, and the glucose consumption and Glut4 expression were detected in each group in 12 h, 24 h, 36 h, 48 h and 60 h. Results Within 24 hours, compared with the normal control group, the glucose consumption in dexamethasone group, free fatty acids group and high glucose and hyperinsulinism group de- creased significantly （P 〈 0.05 ） . After 24 hours, compared with the normal control group, the glucose consumption in free fat- ty acids group showed no statistically significant difference （P 〉 0.05 ） . The glucose consumption in dexamethasone group de- creased significantly compared with normal control group （ P 〈 0.05 ） ; compared with the dexamethasone group, the glucose consumption in high glucose and hyperinsuhnism group decreased significantly （ P 〈 0.05 ） . Compared with the normal control group,the Glut4 expression in dexamethasone group and free fatty acids group both decreased significantly （P 〈 0.01 ）, and compared with dexamethasone group and free fatty acids group, the Glut4 expression in high glucose and hyperinsulinism group decreased significantly （P 〈 0. 01 ） . Conclusion High glucose and hyperinsulinism can induce stronger insulin resistance in 3T3 -L1 adipocytes, and this may result from the inhibition of Glut4 expression.

关 键 词：脂细胞 3T3-L1细胞 胰岛素抵抗 葡萄糖消耗 葡萄糖转运体4型 

分 类 号：R587.1[医药卫生—内分泌]