SSR标记在油菜隐性核不育杂交种的纯度鉴定研究  

Hybrid Purity Identification of Genic Male Sterile by SSR

作　　者：张宁洁[1] 陈丽[1] 朱文秀[1] 曾章丽[1] 王仙萍[1] 

机构地区：[1]贵州省油菜研究所,贵州贵阳550001

出　　处：《安徽农业科学》2012年第11期6376-6377,共2页Journal of Anhui Agricultural Sciences

基　　金：贵州省科研机构创新能力建设项目[黔科合院所(2009)004];贵州省农业科学院基金项目[黔农科合(基金)2010010]

摘　　要：[目的]利用SSR标记对油菜隐性核不育杂交种的纯度进行鉴定。[方法]以贵州省油菜科学研究所选育的甘蓝型油菜"油研系列"为材料,利用简化的SSR标记技术对制种样品进行纯度分析。[结果]筛选出了2对条带清晰、易于统计的SSR引物;大田生产F1杂种群体的纯度鉴定结果表明,SSR鉴定的结果清晰可辨,可信度高,能较准确地反映种子的真实纯度。[结论]该试验为SSR标记在油菜种子纯度鉴定中的进一步应用奠定了基础。[Objective]The aim was to identify the hybrid purity of rape genic male sterile by SSR makers.[Method]To analyze the purity of seed samples by simplified SSR markers,using the Brassica napus ＂Yuyan series＂ as materials which had been nurtured by Guizhou Institute of Oil Crops.[Result]2 primers were selected according to PIC（polymorphism information content） value and the result of F1 purity identification,which produced in field,showed that the SSR method identified quickly and effectively,would provide accurate technical data for the hybrid seed purity identification.[Conclusion]It prepared the ground for the further application of SSR markers in the hybrid seed purity identification of rape.

关 键 词：甘蓝型油菜 杂交种亲本 杂交种纯度 SSR标记 引物筛选 

分 类 号：S564.403.3[农业科学—作物学]