缓冲液匀浆法和丙酮浸提法提取杨桃过氧化酶的研究  

Study on the Extraction of Peroxidase in Carambola(Averrhoa carambola) by Homogenate Buffer and Acetone Extraction

作　　者：景艳艳[1] 杨昌鹏[1,2] 何新华[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西农业职业技术学院,广西南宁530007

出　　处：《安徽农业科学》2012年第11期6486-6487,6575,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为杨桃果实过氧化物酶(POD)的分离纯化提供理论依据。[方法]用缓冲液匀浆法和丙酮浸提法对杨桃POD进行提取,并比较其提取的效果。[结果]用缓冲液匀浆法提取杨桃POD的最佳条件为:PVPP浓度1.0%、提取时间4 h、pH 7.0;用丙酮浸提法提取杨桃POD的最佳条件为:丙酮浓度90%、丙酮浸提时间30 min、料液比1∶4 g/ml。[结论]经比较,用丙酮浸提法提取的杨桃POD的比活性远大于缓冲液匀浆法,约是后者的15倍。在提取杨桃POD时,宜选用丙酮提取法。[Objective] The aim was to provide the theoretical basis for the separation and purification of peroxidase（POD） in carambola fruit.[Method] The POD in carambola was extracted using homogenate buffer and acetone extraction,and the extraction effects were compared.[Result] The optimal conditions for star fruit POD by homogenate buffer were PVPP concentration of 1.0%,extraction time of 4 h,pH of 7.0;and the optimal conditions for star fruit POD by acetone extraction were acetone concentration of 90%,acetone extraction time of 30 min,solid-liquid radio of 1∶4 g/ml.[Conclusion] The acetone extraction was better than homogenate buffer by comparing the two methods,which was about 15 times than the latter.Therefore,the extraction of peroxidase in carambola should select the acetone extraction method.

关 键 词：杨桃 过氧化物酶 提取 

分 类 号：S667.9[农业科学—果树学]