重氮化法合成全抗原的研究  

Study on the Synthesis of Chloramphenicol Complete Antigen Using Diazotization

作　　者：孔令文[1] 周弘春[1] 

机构地区：[1]浙江海洋学院海洋科学学院,浙江舟山316000

出　　处：《安徽农业科学》2012年第11期6568-6569,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]制备氯霉素全抗原,探索氯霉素合成的方法,为动物免疫试验作准备。[方法]采用重氮化法合成氯霉素卵清蛋白全抗原,通过紫外法和红外光谱法测定合成的偶联效果。[结果]重氮化法可用于制备较高偶联率的氯霉素全抗原,用于免疫动物制备抗体。[结论]该研究为合成高效价的抗体奠定了基础。[Objective] The aim was to prepare chloramphenicol complete antigen and explore synthesis methods of chloramphenicol,to provide basis for animal immunology test.[Methods] Diazotization was adopted to synthesize chloramphenicol ovalbumin complete antigen,moreover,the coupling effects of the synthesis were identified using ultraviolet method and infrared spectrum.[Result] Diazotization can be used to prepare chloramphenicol complete antigen with high coupling rate and prepare antibody of immune animals.[Conclusion] The study laid the foundation for the synthesis of high titer antibody.

关 键 词：氯霉素 全抗原 重氮化法 

分 类 号：S851.34[农业科学—预防兽医学] TS254[农业科学—兽医学]