蓝莓对大鼠肝纤维化及肝细胞超微结构的影响  

Effects of blueberry on hepatic fibrosis and ultrastructural of hepatocytes in rats

作　　者：陆爽[1] 程明亮[1] 李宏[1] 吴君[1] 王豫萍[2] 

机构地区：[1]贵阳医学院感染病学教研室,550004 [2]贵阳医学院检验系临床微免教研室,550004

出　　处：《中华医学杂志》2012年第13期927-931,共5页National Medical Journal of China

基　　金：基金项目：贵州省科技厅社会攻关项目（黔科合SY[2010]3017）;贵阳医学院附属医院资助

摘　　要：目的观察蓝莓对大鼠肝纤维化及肝细胞超微结构的影响。方法60只健康清洁级Wistar大鼠随机分为正常对照组（A组）、肝纤维化模型组（B组）、蓝莓原浆低、中、高剂量组（C、D、E组）及复方鳖甲软肝片组（F组），共6组，每组10只。除正常对照组外，其余各组均腹腔注射猪血清制备大鼠肝纤维化模型，C、D、E、F组在造模同时分别给予蓝莓原浆或复方鳖甲软肝片灌胃，1次／d，共12周。12周末处死大鼠，行肝脏HE、Masson染色，并在透射电镜下观察肝组织超微结构变化。计算大鼠肝脏指数、测定血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平、肝组织匀浆超氧化物歧化酶（SOD）活性、丙二醛（MDA）、羟脯氨酸（Hyp）及还原型谷胱甘肽（GSH）含量。结果各组大鼠血清ALT、AST水平差异无统计学意义（P〉0．05）；蓝莓原浆中、高剂量组肝组织匀浆SOD活性、GSH含量明显高于肝纤维化模型组（均P〈0．05），肝脏指数、MDA及Hyp含量明显低于肝纤维化模型组（均P〈0．05）；肝纤维化程度明显减轻（P〈0．05），胶原表达减少，超微结构病变明显改善。结论蓝莓对猪血清所致大鼠肝纤维化有一定的预防作用，对肝细胞线粒体等细胞器损伤有一定的保护作用，以蓝莓原浆中、高剂量组效果较好。其机制可能与其增加肝脏SOD活性及GSH含量，降低MDA含量，提高机体抗氧化应激的能力有关。Objective To observe the effects of blueberry on rat with hepatic fibrosis and uhrastructurah of hepatocytes. Methods Sixty （60） healthy Wistar rats were randomly divided into six groups： normal control group（ group A）, hepatic fibrosis model group（ group B）, blueberry at low, middle and high concentration groups （ group C, D, E）, Fu-Fang-Bie-Jia-Ruan-Gan tablet group （ group F）. The hepatic fibrosis model of rat was established by intraperitoneal injection of porcine serum once daily for 12 weeks. Simultaneously, rats in groups C - F were respectively perfused with blueberry juice or Fu-Fang-Bie- Jia-Ruan-Gan tablet for 12 weeks except for the normal control group which accepted saline alone. Upon terminal sacrifices of all rats at the end of the twelve weeks. Pathology of hepatic tissue was evaluated by hematoxylin-eosin （HE）, Masson staining and transmission electron microscope. Liver index were measured. Levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum were examined. Activities of superoxide dismutase （SOD）, the contents of malondialdehyde （MDA）, hydroxyproline （Hyp） and reduced glutathione （GSH） in liver homogenates were determined. Results Refer to the serum levels of ALT and AST, there is no significant difference existed in all groups （ P 〉 0. 05）. Activities of SOD, the contents of GSH in liver homogenates of group D and E were significantly higher than those of group B（P 〈0. 05 ）, while liver index, the contents of Hyp, MDA in liver homogenates were significantly lower than those of group B （ P 〈 0. 05 ）. Compared with the group B, the pathological stages of hepatic fibrosis in group D and E were significantly reduced （ P 〈 0. 05 ）, the expression of collagen were decreased, uhrastructural alterations were markedly improved. Conclusion Blueberry may have certain preventional protective effects on porcine serum induced rat hepatic fibrosis and exhibit certainprotective effects on organelles of

关 键 词：肝硬化 肝细胞 蓝莓 

分 类 号：R285.5[医药卫生—中药学]