羊大片吸虫龙岩分离株ITS基因的克隆及序列分析  

PCR Amplification,Cloning and Sequencing of ITS rDNA of Fasciola Gigantica from Longyan

作　　者：谢宝明[1] 陈永锋 陈仁锋[1] 黄翠琴[1,3] 

机构地区：[1]龙岩学院,福建龙岩364012 [2]厦门市农产品质量安全检验测试中心,福建厦门361000 [3]福建省人畜寄生与病毒性疫病防控工程技术研究中心,福建龙岩364000

出　　处：《龙岩学院学报》2012年第2期61-64,共4页Journal of Longyan University

基　　金：福建省科技计划项目(2008N2005);福建省高等学校杰出青年科研人才培育计划资助项目(2011)

摘　　要：应用PCR方法扩增龙岩地区羊片形吸虫分离株ITS序列,经克隆、测序后获得ITS全长序列944bp,其中ITS-1为421bp,ITS-2为361bp;通过在肝片吸虫和大片吸虫ITS种间鉴别位点上的序列分析表明,从龙岩地区羊体内分离的片形吸虫虫种属大片吸虫(命名为FgLY)。与国内外大片吸虫的进化分析表明,FgLY与中国云南的2个分离株处在一个小分支,亲缘关系最近。该结果为羊大片吸虫的进一步生物学研究和片形吸虫病的预防奠定了基础。In the paper,the samples of Fasciola（Platyhelminthes： Trematoda： Digenea） from sheep host in Longyan,Fujian,China were characterized genetically by sequences of the first（ITS-1） and second（ITS-2） internal transcribed spacers（ITS） of nuclear ribosomal DNA（rDNA）.The ITS rDNA was amplified by polymerase chain reaction（PCR）,and the amplification was cloned and then sequenced.The lengths of the ITS-1 and ITS-2 sequences were 421 bp and 361 bp respectively.Comparison of the ITS sequences of Longyan Fasciola sample with that of Fasciola hepatica,Fasciola gigantica and the ＂intermediate Fasciola＂ revealed that Longyan Fasciola samples examined represent F.gigantica（named FgLY）.The genetic relationship of FgLY is closest to the samples from Yunnan,China by phylogenetic analysis.The result has laid foundation for studying the population genetic structure of F.gigantica and for the diagnosis and control of the disease it causes.

关 键 词：大片吸虫 ITS PCR 序列分析 

分 类 号：S858.26[农业科学—临床兽医学]