碳纤维纳米电极用于微流控芯片安培测定单个PC12细胞中神经递质多巴胺  

Carbon Fiber Nanoelectrodes Applied to Microchip Electrophoresis Amperometric Detection of Neurotransmitter Dopamine in Single Rat Pheochromocytoma Cells

作　　者：程寒[1] 袁琳[1] 明月[1] 杨天鸣[1] 程介克[2] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《中南民族大学学报（自然科学版）》2012年第1期25-29,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学中央高校基本科研业务费专项资金项目(ZZQ10012)

摘　　要：为研究单个PC12细胞中多巴胺的含量,以碳纤维纳米电极为工作电极,自行组装了微流控芯片安培柱末检测系统,设计了一种微流控芯片电泳单细胞进样和溶膜的控制方式,仅需一路高压控制即可实现单细胞进样和溶膜操作.结果表明:采用该系统实现了4种儿茶酚胺类物质的基线分离,通过控制适当的细胞密度及液池的液面高度差,实现了单个细胞连续快速进样、溶膜和分析,得出单个PC12细胞中多巴胺含量为0.80±0.33 fmol(n=5).A microchip electrophoresis system with end-channel carbon fiber nanoelectrode amperometric detector was developed to study on the content of DA in single phochromocytoma（PC12） cell.An operation mode was designed to achieve single cell injection and lysis in chip-CE with only one high-voltage power supply.The experimental results indicate that four catecholamines were baseline-separated and determined with this system.The cell density and liquid height of the reservoirs were accommodated for single cell loading,docking and analysis.The microchip CE system has been successfully applied to the determination of dopamine（DA） in single cultured rat PC12 cell and the average content of DA in single PC12 cell is 0.80±0.33（n=5） fmol.

关 键 词：芯片毛细管电泳 电化学检测 碳纤维纳米电极 PC12细胞 多巴胺 

分 类 号：O657.1[理学—分析化学]