血清中白细胞介素及肿瘤坏死因子-α含量与幽门螺杆菌感染的关系  

Relationship between interleukins and tumor necrosis factor-α and Helicobacter pyloriinfections

作　　者：卢灿荣[1] 孙刚[2] 周智勇[2] 张勇[1] 卫勃[1] 

机构地区：[1]解放军总医院普通外科,北京100853 [2]解放军总医院消化内科,北京100853

出　　处：《中华医院感染学杂志》2012年第8期1552-1554,共3页Chinese Journal of Nosocomiology

基　　金：北京市科技新星计划(2009A38)

摘　　要：目的探讨白细胞介素-4(IL-4)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量的变化与胃幽门螺杆菌(HP)感染之间的关系。方法采用Luminex 100液态芯片仪检测血清中5种细胞因子的含量,HP检测使用快速尿素酶法和14 C尿素呼气试验,然后按照胃窦黏膜炎症组织学分级标准将炎症分为不同等级。结果 5种细胞因子中,IL-4、IL-8和TNF-α在HP阴性组和阳性组之间差异有统计学意义(P<0.05),IL-4、IL-8和TNF-α在HP阳性组含量分别为(138.23±12.38)、(286.59±82.51)、(37.61±19.28)pg/ml,而IL-10、IL-1β差异无统计学意义;将HP阳性组分为轻度、中度和重度组,与轻度组相比较,中度组和重度组的IL-8、TNF-α含量均显著升高;与中度组相比较,轻度组和重度组的IL-8、TNF-α含量差异均有统计学意义(P<0.05);IL-8在轻、中、重度组的含量分别为(213.78±13.19)、(241.02±20.62)、(319.83±40.92)pg/ml,TNF-α在轻、中、重度组的含量分别为(24.01±7.39)、(29.85±10.27)、(44.71±11.82)pg/ml;IL-4在不同炎症程度组差异无统计学意义,而IL-8和TNF-α随着炎症程度升高含量也升高,且差异有统计学意义(P<0.05)。结论 IL-4、IL-8和TNF-α与胃幽门螺杆菌感染密切相关,且IL-8和TNF-α的含量与炎症程度具有一定的相关性。OBJECTIVE To investigate the relationship between interleukin-4,IL-8,interleukin-10,IL-1β and tumor necrosis factor-α and Helicobacter pylori infection.METHODS The 5 kinds of cytokines in serum were detected by Luminex 100.H.pylori were detected by the rapid urease enzyme and 14C urea breath test.According to the histological grading of gastric mucosal inflammation standard,the inflammation were divided into different levels.RESULTS In the five kinds of cytokines,IL-4,IL-8 and TNF-α showed significant difference between the HP-negative group and HP-positive group（P〈0.05）.The concentration of IL-4,IL-8 and TNF-α in the HP-positive group were separately（138.23±12.38）,（286.59±82.51）,（37.61±19.28）pg/ml.The IL-10 and IL-1β showed no significant difference.The HP-positive group was divided into mild,moderate and severe groups according to histological grading.Compared with the mild group,IL-8 and TNF-α levels in the moderate and severe group showed significantly increased.Compared with the moderate group,IL-8 and TNF-α levels in the mild and severe group showed statistically significant difference.The concentrations of IL-8 in the mild,moderate and severe groups were separately（213.78±13.19）,（241.02±20.62）and（319.83±40.92）pg/ml.The concentrations of TNF-α in the mild,moderate and severe groups were separately（24.01±7.39）,（29.85±10.27） and（44.71±11.82）pg/ml.The results indicated that IL-4 showed no significant difference in the different degree of inflammation group,and IL-8 and TNF-α levels increased when the degree of inflammation increased,and the difference was statistically significant（P〈0.05）.CONCLUSION IL-4,IL-8 and TNF-α show closely relationship with the H.pylori infection.IL-8 and TNF-α levels are closely related to the degree of inflammation.

关 键 词：幽门螺杆菌 慢性胃炎 白细胞介素 肿瘤坏死因子-Α 

分 类 号：R378[医药卫生—病原生物学]