穿心莲内酯和脱水穿心莲内酯大孔树脂纯化工艺  

Purification Technology of Andrographolide and Dehydroandrographolide by HPD100

作　　者：涂瑶生[1] 付建武[1,2] 孙冬梅[1] 张建军[1] 江志强[1,2] 李绍林[1,2] 谢菲 

机构地区：[1]广东省中医研究所,广州510095 [2]广州中医药大学,广州510405 [3]广东一方制药有限公司,广东佛山528244

出　　处：《中国实验方剂学杂志》2012年第8期22-25,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究穿心莲内酯和脱水穿心莲内酯的大孔树脂吸附分离纯化工艺。方法:考察HPD100型大孔树脂的吸附性能和洗脱参数,采用HPLC测定穿心莲内酯和脱水穿心莲内酯含量。结果:HPD100型大孔树脂适宜的工艺条件为最大上样量为每克药材1.0 g树脂,5 BV 20%乙醇除杂,10 BV 35%乙醇,5 BV 55%乙醇溶液分别洗脱,总内酯纯度68.46%;35%乙醇洗脱液中主要富含穿心莲内酯;55%乙醇洗脱液中富含脱水穿心莲内酯。结论:HPD100型大孔吸附树脂在所确定的工艺条件下,对穿心莲内酯和脱水穿心莲内酯的纯化效果良好,对大生产有指导意义。Objective：To study on separation and purification technology of andrographolide and dehydroandrographolide by macroporous resin.Method： Based on screening model of resin,characteristics of adsorbing and eluting parameter had been obtained by HPD100 resin,and the content of andrographolide and dehydroandrographolide were determined by HPLC.Result： Optimum parameter of purification were as follows： maximum sample volume was 1.0 g of resin per medicine material,5 BV 20% ethanol was used for eluting impurity,for eluting target components was 10 BV 35% ethanol（for andrographolide） and 5 BV 55% ethanol（for dehydroandrographolide）.Purity of andrographolide and dehydroandrographolide were respectively 48.59% and 19.87% and total lactone was 68.46%.Conclusion： HPD100 resin was favorable to andrographolide and dehydroandrographolide under condition of determined process,and it had significance for large-scale production.

关 键 词：穿心莲内酯 脱水穿心莲内酯 HPD100型大孔树脂 纯化 

分 类 号：R283.6[医药卫生—中药学]